Development of a Quasi-Steady Flow Electrochemical Paper-Based Analytical Device

Details

• Personal Author:
  Adkins JA ; Henry CS ; Noviana E
• Description:
  An electrochemical paper-based analytical device (ePAD) was developed for quasi-steady flow detection at microwire electrodes, for the first time. The device implements a fan shaped geometry connected to an analysis channel whereby solution is pulled from an inlet, through a channel, and into the steadily increasing capillary network of the fan. The network counteracts the decrease in solution flow rate associated with increasing viscosity within the channel, generating quasi-steady flow within the analysis channel. Microwire electrodes were embedded between two paper layers within the analysis channel, such that solution flow occurred on both sides of the wire electrodes. The quasi-steady flow ePAD increased the current by 2.5 times and 0.7 times from a saturated channel with no flow and from a single-layer paper device with flow, respectively. Amperometric detection was used for flow injection analysis (FIA) of multiple analytes at both Au and Pt microwire working electrodes, both of which provided similar sensitivity (ca. 0.2 mM-1) when normalized to the same standard. The two-layer paper devices provided a detection limit of 31 µM for p-aminophenol (PAP) using Pt electrodes and was also used to detect enzyme activity for the reaction of beta-galactosidase with p-aminophenyl-galactopyranoside (PAPG). Measured enzyme kinetics provided similar Vmax (0.079 mM/min) and Km (0.36 mM) values as those found in the literature. This device shows great promise toward use in enzyme-linked immunosorbent assays or other analytical techniques where flow or washing steps are necessary. The developed sensor provides a simple and inexpensive device capable of performing multiple injection analysis with steady-flow and online detection that would normally require an external pump to perform. [Description provided by NIOSH]
• Subjects:
  Chemistry, Analytic Enzymes Laboratories Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Instruments Analytical Processes Detectors Electrical Conductivity Electrochemical Properties Electrochemical Reactions Fluids Laboratory Techniques Nanoparticles Nanoscale Oxidation Oxidation Reduction Reactions Sensors Viscosity Volumetric Analysis

  More +
• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  88
• Issue:
  21
• NIOSHTIC Number:
  nn:20052567
• Citation:
  Anal Chem 2016 Nov; 88(21):10639-10647
• Contact Point Address:
  Charles S. Henry, Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States
• Email:
  Chuck.Henry@colostate.edu
• Federal Fiscal Year:
  2017
• Performing Organization:
  Colorado State University, Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20150901
• Source Full Name:
  Analytical Chemistry
• End Date:
  20190831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:31a6d2c518b0e338ec0fa02485221df79cff9fbb05f1fbd0ccc9c3e2325a64fa581c89a870513d01457d7e445e17241f9ff403595bd5647a6459636cc69972c0
• Download URL:
  https://stacks.cdc.gov/view/cdc/212078/cdc_212078_DS1.pdf
• File Type:
  [PDF - 2.63 MB ]