Prevalence of Escherichia coli O157 Shedding in Preweaned Calves on Colorado Dairies

Details

• Personal Author:
  Hyatt DR ; Linke L ; Magnuson R ; McConnel C ; Stenkamp-Strahm C ; Tenneson P
• Description:
  To gain insight into a potential age-related predisposition for Escherichia coli pathogen shedding on dairies, this pilot study measured the prevalence of E. coli O157 (ECO157) in the feces of preweaned dairy calves. An aim of this study was to link these outcomes with the concurrent environmental presence of ECO157 and dam ECO157 shedding elucidated in a parallel study. Recto-anal mucosal swabs and a subset of fecal grab samples were collected from calves (2 to 8 weeks of age; n = 399) monthly between December 2013 and June 2014 on three dairies in northern Colorado. A subset of calf dams (n = 111) were also sampled via fecal grab. Concurrently, environmental samples were collected from locations within the vicinity of the calves: farm tractor tires, steering wheels, hutches, buckets, and gloves from the research technicians and the employees involved in calf rearing. The presence of ECO157 and virulence genes was measured in the samples and confirmed via PCR. Of the calves, only 1 (0.25%) of 399 individuals shed during the time period, and the ECO157 strain detected carried no measured virulence genes (eaeA, stx1, and stx2). No difference was seen in detection between the recto-anal mucosal swabs and the fecal grab technique. In contrast, 32% (35 of 111) of the dams shed ECO157, with 1.8% (2 of 111) of the shed isolates containing virulence genes. No ECO157 was detected in the environmental samples. These outcomes demonstrate a disparity between dam and calf ECO157 shedding and indicate that preweaned calves, managed similarly to those of this study, probably have a minor influence on dairy contamination and the transmission of ECO157. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Dairy Products Livestock Microbiology Occupational Health Safety
• Keywords:
  Agricultural Industry Animal-related Diseases Animal Waste Author Keywords: Calves Bacterial Disease Cattle Dairy Dairy Products Food Contaminants Microorganisms O157 Pathogens Shedding

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• ISSN:
  0362-028X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 10 ; OSHA Region 8 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  80
• Issue:
  6
• NIOSHTIC Number:
  nn:20051887
• Citation:
  J Food Prot 2017 Jun; 80(6):990-993
• Contact Point Address:
  Chloe Stenkamp-Strahm, Animal Population Health Institute, Department of Clinical Sciences, Colorado State University, Fort Collins, Colorado 80523-1644
• Email:
  cstenkampstrahm@gmail.com
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  True
• Start Date:
  20030915
• Source Full Name:
  Journal of Food Protection
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:972128fb1e8504329ef05eea0798c5aca02289d35d25eb5b376f93f9f50a807418bdbfd42fbf91e60d6f944cbb21e140c550f5688769bdc8fa6f4521445066ea
• Download URL:
  https://stacks.cdc.gov/view/cdc/211569/cdc_211569_DS1.pdf
• File Type:
  [PDF - 66.01 KB ]