Associations Between Escherichia coli O157 Shedding and the Faecal Microbiota of Dairy Cows

Details

• Personal Author:
  Abdo Z ; Magzamen S ; McConnel C ; Reynolds S ; Stenkamp-Strahm C
• Description:
  Aims: Dairy cattle shed pathogenic Escherichia coli O157 (O157) in faeces, playing a role in human exposure. We aimed to measure faecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. Methods and Results: Daily faecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene sequencing was used to measure sample-level microbial communities. Alpha-diversity metrics were associated with O157 outcomes via regression modelling, adjusting for confounders. Differential abundance of taxa were identified between O157(+) and O157(-) samples and between shedding days of individuals, using matched Wilcoxon rank-sum tests, zero-inflated Gaussian (ZIG) regression and negative binomial regression. After removing an outlier, multi-day and intermittently shedding cows had lower average richness compared to those that never shed. ZIG modelling revealed Bacillus coagulans to be more abundant in O157(-) samples, while Moryella were more abundant in O157(+) samples. Negative binomial models and Wilcoxon tests revealed no differentially abundant taxa between O157(+) vs O157(-) samples, or between shedding days of individuals. Conclusions: Microbial diversity and some taxa may be influenced by or affect O157 shedding by dairy cattle. Significance and Impact of the Study: If future work corroborates these findings, dairy cow microbial community changes may be used to guide on-farm strategies that mitigate O157 dissemination, protecting the human food chain. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Dairy Products Livestock Microbiology Occupational Health Safety
• Keywords:
  Animal Husbandry Workers Animal Waste Bacterial Infections Cattle Dairy Products Food Contaminants Microbial Test Systems Microorganisms Pathogens
• ISSN:
  1364-5072
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  124
• Issue:
  3
• NIOSHTIC Number:
  nn:20051374
• Citation:
  J Appl Microbiol 2018 Mar; 124(3):881-898
• Contact Point Address:
  Chloe Stenkamp-Strahm, CVMBS, Colorado State University, 1644 Campus Delivery, Fort Collins, CO 80523, USA
• Email:
  cstenkam@lamar.colostate.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  True
• Start Date:
  20030915
• Source Full Name:
  Journal of Applied Microbiology
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a09c62bd77ec25ffc29078769153a15fe0b8620901319bc29b908e18d8f8ae6e5c8c19e05cf2a56117d576bcff15d0ea58d5b8c71803a73ff2b773b0c86bfd2b
• Download URL:
  https://stacks.cdc.gov/view/cdc/211366/cdc_211366_DS1.pdf
• File Type:
  [PDF - 1.35 MB ]