Involvement of ROS Generation in Copper Oxide Nanoparticles-Induced AP-1 Activation

Details

• Personal Author:
  Aldinger, Jeffrey A. ; Ding M ; Tabatha B ; Zhao J
• Description:
  Occupational exposures to copper dusts or fumes have been reported to be harmful to human health, with possible risk of cancer among copper smelter workers. Copper (II) oxide (CuO) nanoparticles have not, to our knowledge, been extensively examined for potential carcinogenic or genotoxic effects. To investigate the mechanisms of CuO-induced pathogenesis, the effect of CuO on AP-1-MAPKs and ROS generation were investigated. The results indicated CuO caused a 2-fold increase in AP-1 activity in JB6 cells. The induction of AP-1 activity in cultured cell lines was time and dose-dependent. The signal transduction pathways for AP-1 activation were also investigated. Western Blot analysis demonstrate that CuO stimulates phosphorylation of p38 MAPK and ERKs. CuO also generated ROS when incubated with the cells as measured by electron spin resonance (ESR). Nano-sized CuO generated more ROS than the fine sized particles when incubated with the cells. Finally, co-incubation of the cells with free radical scavenger N-acetylcysteine or PVPNO decreased AP-1 activation and phosphorylation of MAPKs, thus suggesting that oxidative stress is involved in WC-Co-induced toxicity and AP-1-MAPKs activation. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Free Radicals Occupational Health Safety Toxicology
• Keywords:
  Cell Cultures Cell Differentiation Cellular Effects Cellular Reactions Copper Dust Copper Fumes Copper Oxide Electron Spin Resonance ESR Free Radicals Gene Expression Gene Regulation Nanoparticles Nanotechnology Nanotoxicology Oxidative Phosphorylation Oxidative Stress Pathogenesis Proteins Reactive Oxygen Species ROS Toxic Effects

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  162
• Issue:
  1
• NIOSHTIC Number:
  nn:20051246
• Citation:
  Toxicologist 2018 Mar; 162(1):409
• CAS Registry Number:
  Copper oxide (CuO) (CAS RN 1317-38-0) ; N-Acetyl-L-cysteine (CAS RN 616-91-1)
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 57th Annual Meeting and ToxExpo, March 11-15, 2018, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fc60a8a95a8e05996c756906e5941e10e9aeffeb182c8f3f7c4b3f3d07f18625ccba5dfb5cfac0d58ddfe2343ae136da92be81bfafa70e1d8b90db0633c7a302
• Download URL:
  https://stacks.cdc.gov/view/cdc/211312/cdc_211312_DS1.pdf
• File Type:
  [PDF - 838.97 KB ]