An Enzyme-Linked Immunosorbent Assay for Detecting 3-Phenoxybenzoic Acid in Plasma and Its Application in Farmers and Consumers

Details

• Personal Author:
  Ahn KC ; Chantara S ; Gee SJ ; Hammock BD ; Mangklabruks A ; Prapamontol T ; Suphavilai C ; Thiphom S
• Description:
  The aim of this study was to identify a plasma biomarker of exposure to pyrethroid insecticides. A major metabolite, 3-phenoxybenzoic acid (3-PBA), can be detected in urine but urinary 3-PBA cannot be used to assess the active dose. The 3-PBA-adduct represents a much more persistent class of biomarkers than metabolites excreted into urine, having half lives up to several weeks or months. We developed an enzyme-linked immunosorbent assay (ELISA) for total 3-PBA including adduct formed after alkaline hydrolysis, liquid-liquid extraction (LLE) and solid phase extraction (SPE) of the sample. The developed ELISA had an IC50 value of 26.7 ng/mL. The intra- and inter-assay coefficients of variation (%CV) were lower than 5% and were within the optimum condition variance (OCV) range. The LLE cleanup technique satisfactorily eliminated the matrix effect from plasma samples before SPE and ELISA analysis yielding good recoveries (85.9-99.4%) with a limit of quantitation (LOQ, 5 ng/mL) that was 30- to 47-fold more sensitive than previous studies. Moreover, the developed method could separate more than 80% of 3-PBA from adduct form. The method was successfully applied to the detection of the target in real samples obtained from consumers (n=50) and farmers (n=50). To our knowledge, this is the first ELISA method for detecting 3-PBA in human plasma and applied to a field study. [Description provided by NIOSH]
• Subjects:
  Acids Agriculture Biological Assay Biomarkers Blood Energy Metabolism Enzymes Farmers Insecticides Occupational Health Safety Urine

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• Keywords:
  3-phenoxybenzoic Acid Agricultural-chemicals Analytical Methods Author Keywords: Enzyme-linked Immunosorbent Assay Bioassays Biological Monitoring Blood-plasma Consumer ELISA Enzyme-linked Immunosorbent Assay Farmer Humans Hydrolysis Metabolites Performance Capability Plasma Pyrethroid Insecticides Pyrethroids Sampling Statistical-analysis Urinalysis Urine Chemistry

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• ISSN:
  1759-9660
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  11
• NIOSHTIC Number:
  nn:20051158
• Citation:
  Anal Methods 2012 Oct; 4(11):3772-3778
• Contact Point Address:
  Tippawan Prapamontol, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
• Email:
  tprapamontol@rihes.org
• CAS Registry Number:
  3-Phenoxybenzoic acid (CAS RN 3739-38-6) ; λ-Cyhalothrin (CAS RN 91465-08-6)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Analytical Methods
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:89fcae796b88087888522e9728a40a96427ce165f1f62c12dd4876d6a296e14cf8a4a857b4fd79febd0814a30ebd08746f4acfa6ea5f1bdc59b8910dff1066df
• Download URL:
  https://stacks.cdc.gov/view/cdc/211257/cdc_211257_DS1.pdf
• File Type:
  [PDF - 186.13 KB ]