An Immunoassay for a Urinary Metabolite as a Biomarker of Human Exposure to the Pyrethroid Insecticide Permethrin

Details

• Personal Author:
  Ahn KC ; Gee SJ ; Hammock BD ; Ma S-J ; Tsai H-J
• Description:
  Permethrin is the most popular synthetic pyrethroid insecticide used in agriculture and public health. For the assessment of human exposure to permethrin, a competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the glycine conjugate of a major metabolite, cis-/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DCCA), of permethrin was developed based on a polyclonal antibody. An assay based on an antibody with a high sensitivity was optimized and characterized. The IC50 value and the detection range for trans-DCCA-glycine, in the assay buffer were 1.2 and 0.2-7.0 microg/L, respectively. The antibody recognized trans-DCCA-glycine and the mixture of cis-/trans-DCCA-glycine with an isomer range from 30:70 to 50:50 nearly equally. Little or no cross-reactivity to permethrin and its other free metabolites or glycine conjugates was measured. The integration of the ELISA and solid-phase extraction which was used to reduce the matrix effect from human urine samples provided for analysis of total cis-/trans-DCCA-glycine at low parts per billion levels in the samples. The limit of quantitation of the target analyte was 1.0 microg/L in urine with a limit of detection of 0.1 microg/L in buffer. This assay might be a useful tool for monitoring human exposure to permethrin. [Description provided by NIOSH]
• Subjects:
  Agriculture Biological Assay Biomarkers Enzymes Insecticides Occupational Exposure Occupational Health Safety Urine
• Keywords:
  Author Keywords: ELISA Bioassays Biological Monitoring ELISA Enzyme-linked Immunosorbent Assay Glycine Metabolite Human Exposure Occupational Exposure Permethrin Polyclonal Antibodies Urinalysis Urine Chemistry

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• ISSN:
  1618-2642
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Kentucky ; OSHA Region 4 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  384
• Issue:
  3
• NIOSHTIC Number:
  nn:20031881
• Citation:
  Anal Bioanal Chem 2006 Feb; 384(3):713-722
• Contact Point Address:
  Bruce D. Hammock, Department of Entomology and UCD Cancer Research Center, University of California, Davis, CA 95616
• Email:
  bdhammock@ucdavis.edu
• CAS Registry Number:
  Permethrin (CAS RN 52645-53-1)
• Federal Fiscal Year:
  2006
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Analytical and Bioanalytical Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e0249b5aa3f34ee72b4935c88425fda60bfdbd2e5ecb2d8bb302cd1ce13ea88435a2693622e0277890c5655d3f57126e3e0206431711afa32fbe9af90ede80df
• Download URL:
  https://stacks.cdc.gov/view/cdc/185997/cdc_185997_DS1.pdf
• File Type:
  [PDF - 206.07 KB ]