Characterization and Normalization of Cholinesterase Activity in Rat Saliva

Details

• Personal Author:
  Klohe EM ; Lee, Stephen A. ; Smith JN ; Timchalk C
• Description:
  Cholinesterases (ChE) are enzymes that catalyze the hydrolysis of carboxylic esters, such as the neurotransmitter acetylcholine. Organophosphate (OP) pesticides, which are commonly used in agriculture, inhibit ChE activity, and ChE inhibition in plasma and red blood cells is used routinely as a biomarker for human OP exposure. Currently, salivary ChE activity is being explored as a potential non-invasive biomarker for OP exposure, however little is known about baseline ChE activity. In order to characterize baseline salivary ChE, saliva was collected from 5 adult male Sprague-Dawley rats over 2 h, salivary ChE was measured using the Ellman assay, and total salivary protein was measured using the bicinchoninic acid (BCA) protein assay. Comparison of total ChE activity indicated that rat salivary ChE is variable among individual rats at each time point of collection (coefficient of variation (CV) ranging 33-65%) and over the entire duration of collection for each rat (CV ranging 16-104%). Overall CV for all rats over the entire collection time was 67%. Variation of total protein in saliva was also observed among individuals at each collection time (CV ranging 36-57%) and over the duration of collection for each individual (CV ranging 15-105%) as well. Protein content and total ChE activity were correlated (0.73 correlation coefficient, p < 0.001), and normalization of ChE activity by total protein resulted in a CV reduction to 37% for all rats over the entire collection time. These results demonstrate the variability of salivary ChE activity and suggest that salivary protein may be useful to normalize ChE activity in saliva for biomonitoring purposes. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Biomarkers Blood Environmental Exposure Enzymes Insecticides Laboratories Occupational Health Organophosphates Safety

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• Keywords:
  Biomonitoring Blood Cells Exposure Levels Hydrolysis Laboratory Animals Organophosphorus Pesticides Proteins Statistical Analysis
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Montana ; OSHA Region 10 ; OSHA Region 8 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20050715
• Citation:
  49th Annual Meeting and ToxExpo of the Society of Toxicology, March 7-11, 2010, Salt Lake City, Utah. Reston, VA: Society of Toxicology, 2010 Mar; :644
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  False
• Start Date:
  20060901
• Source Full Name:
  49th Annual Meeting and ToxExpo of the Society of Toxicology, March 7-11, 2010, Salt Lake City, Utah
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a762ef5a5852ee8ee324e61ec462d373794e6d27e010e5b656a4893c9f652c69e218438d500707f7a0a0aa2afec937f0e8599f8f8a6cb787dd10d08f0cd0f0a4
• Download URL:
  https://stacks.cdc.gov/view/cdc/210954/cdc_210954_DS1.pdf
• File Type:
  [PDF - 5.34 MB ]