Carbon Nanotube-Based Electrochemical Sensor for Assay of Salivary Cholinesterase Enzyme Activity: An Exposure Biomarker of Organophosphorus Pesticides

Details

• Personal Author:
  Du D ; Lin Y ; Timchalk C ; Wang J
• Description:
  Certain saliva enzymes may be useful biomarkers for detecting exposures to organophosphorus pesticides. Saliva biomonitoring offers a simple approach for rapidly evaluating those exposures in real time. In this regard, an electrochemical sensor coupled with a micro- flow injection system is developed for rapid and sensitive characterization of cholinesterase enzyme (ChE) activities in rat saliva. The electrochemical sensor is based on a carbon nanotube (CNT)-modified screen printed carbon electrode (SPE), which is integrated into a flow cell. Because of the excellent electrocatalytic activity of the CNTs, the sensor can detect electroactive species that are produced from enzymatic reactions with extremely high sensitivity and at low potentials. The sensor is very sensitive to varied AChE concentrations with a detection limit down to 2 pM. Then, the inhibited ChE by organophosphorus agent can be completely reactivated by introducing nucleophiles such as pralidoxime iodide. This oxime-induced reactivation is the primary therapeutic approach to organophosphorus poisoning. Based on this double test of enzyme activity, a new rapid and reliable method for determination of trace organophosphorus exposure is proposed. This approach is very valuable and represents a new field for the improvement of diagnosis of exposure to organophosphorus pesticides. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Environmental Exposure Enzymes Insecticides Laboratories Occupational Health Organophosphates Poisoning Safety
• Keywords:
  Exposure Levels Laboratory Animals Organophosphorus Compounds Organophosphorus Insecticides Organophosphorus Pesticides Pesticides Poisons Sensors
• Publisher:
  American Chemical Society
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20050550
• Citation:
  American Chemical Society 64th Northwest Regional Meeting, June 28-July 1, 2009, Tacoma, Washington. Washington, DC, American Chemical Socitey, 2009 Jun; :109
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  False
• Start Date:
  20060901
• Source Full Name:
  American Chemical Society 64th Northwest Regional Meeting, June 28-July 1, 2009, Tacoma, Washington
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8d010f159668d2f600d95208001cde663fb0847adc6331b074c5bcf10145bde6b7ea22fbdf24473cea286b19b067dacfec257084cda609bfd36910f0c7727539
• Download URL:
  https://stacks.cdc.gov/view/cdc/210853/cdc_210853_DS1.pdf
• File Type:
  [PDF - 155.99 KB ]