Immunoassays of Phosphorylated Cholinesterase as Biomarkers for Rapid Diagnosis of Exposure to Organophosphorous Agents

Details

• Personal Author:
  Lin C ; Lin Y ; Pinwattana K ; Tang Z ; Timchalk C ; Wang J ; Wu H ; Zhang Y
• Description:
  Numerous analysis methods for effectively monitoring neurotoxic organophosphates (OPs) have been developed to assess OP exposures. Biomonitoring of OP exposures is recognized to be one of the best approaches. Among these, enzyme linked immunosorbent assay (ELISA) and Western blot (WB) methods are simple, rapid detection methods for biomarkers. In this work, we used ELISA and WB methods to find specific antibodies for phosphorylated acetylcholinesterase (OP-AchE) and bcetylcholinesterase (OP-BchE) and evaluate their specificity and sensitivity for rapid diagnosing the exposure to OP agents. Our results indicate that anti-phosphoserine antibody can specifically bind to OP moiety of phosphorylated ChE and don't bind to unmodified ChE. Furthermore, couples the AchE or BchE antibody with anti-phosphoserine antibody can be used for direct detecting OP-AchE or OPBchE that prepared with paraoxon as an OP model agent with sandwich immunoassay method. Low detection limit were obtained. Such immunoassay holds great promise as a simple, selective and sensitive for the effective biomonitoring and diagnosis of potential exposures to OP agents. [Description provided by NIOSH]
• Subjects:
  Antibodies Biomarkers Environmental Exposure Enzymes Occupational Health Risk Assessment Risk Factors Safety
• Keywords:
  Biomonitoring ELISA Enzyme-linked Immunosorbent Assay Exposure Levels Immunoassays Monitoring Systems Organophosphorus Compounds Risk Factors
• Publisher:
  American Chemical Society
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20050714
• Citation:
  American Chemical Society 64th Northwest Regional Meeting, June 28-July 1, 2009, Tacoma, Washington. Washington, DC: American Chemical Socitey, 2009 Jun; :175
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  False
• Start Date:
  20060901
• Source Full Name:
  American Chemical Society 64th Northwest Regional Meeting, June 28-July 1, 2009, Tacoma, Washington
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:503bdec244c4c420ce336a8e1592269d869491b5679030202d63708d6bbc67b91dcd13fc0a4106e1de3c14488a5530808f26ad427232c615856e531f7533b84c
• Download URL:
  https://stacks.cdc.gov/view/cdc/210953/cdc_210953_DS1.pdf
• File Type:
  [PDF - 57.91 KB ]