Enzyme-Linked Immunosorbent Assay for Detection of Organophosphorylated Butyrylcholinesterase: A Biomarker of Exposure to Organophosphate Agents

Details

• Personal Author:
  Du D ; Lin C-T ; Lin Y ; Liu F ; Lu D ; Smith JN ; Timchalk C ; Wang J ; Wang L
• Description:
  A sandwich enzyme-linked immunosorbent assay (sELISA) has been developed for detection of organophosphorylated butyrylcholinesterase (OP-BChE), a potential biomarker for human exposure to organophosphate insecticides and nerve agents. A pair of antibodies specific to OP-BChE adduct were identified through systematic screening of several anti BChE antibodies (anti-BChE) and anti-phosphoserine antibodies (anti-P(ser)) from different sources. The selected anti-BChE (set as capture antibody) antibodies recognize both phosphorylated and nonphosphorylated BChE. These antibodies can therefore be used to capture both BChE and OP-BChE from the sample matrices. The anti-P(ser) (set as detecting antibody) was used to recognize the OP moiety of OP-BChE adducts. With the combination of the selected antibody pair, several key parameters (such as the concentration of anti-BChE and anti-P(ser), and the blocking agent) were optimized to enhance the sensitivity and selectivity of the sELISA. Under the optimal conditions, the sELISA has shown a wide linear range from 0.03 nM to 30 nM, with a detection limit of 0.03 nM. Furthermore, the sELISA was successfully applied to detect OP-BChE using in vitro biological samples such as rat plasma spiked with OP-BChE with excellent adduct recovery (z>99%). These results demonstrate that this novel approach holds great promise to develop an ELISA kit and offers a simple and cost-effective tool for screening/evaluating exposure to organophosphate insecticides and nerve agents. [Description provided by NIOSH]
• Subjects:
  Animals Antibodies Biomarkers Blood Environmental Exposure Enzymes Hematologic Tests Immune System Insecticides Laboratories Occupational Health Risk Assessment Risk Factors Safety

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• Keywords:
  Author Keywords: Sandwich ELISA Biological Factors Blood Samples Butyrylcholinesterase ELISA Enzyme-linked Immunosorbent Assay Exposure Levels Immunology In Vitro Study Laboratory Animals Organophosphate Insecticides And Nerve Agents Organophosphorus Compounds Organophosphorylated Risk Factors Sampling

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• ISSN:
  0003-2670
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-6
• Volume:
  693
• Issue:
  1
• NIOSHTIC Number:
  nn:20050537
• Citation:
  Anal Chim Acta 2011 May; 693(1-2):1-6
• Contact Point Address:
  Fengquan Liu, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
• Email:
  fqliu20011@sina.com
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Analytica Chimica Acta
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8d79c8904bd0161a775cb646d14365ce1f74a3d7b0ab10331ae81bc25fe54775b12391c1c94d8ef48b5c486eba22e11fa2cbb9798f6da7cc8b46b1f1f02de908
• Download URL:
  https://stacks.cdc.gov/view/cdc/210844/cdc_210844_DS1.pdf
• File Type:
  [PDF - 585.86 KB ]