Development of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Analysis of 6-Benzylaminopurine and Its Ribose Adduct in Bean Sprouts

Details

• Personal Author:
  Guo S ; He L ; Li QX ; Ning X ; Tan G ; Wang B ; Yue H ; Zhang R ; Zhang W
• Description:
  6-Benzylaminopurine (6-BA), a cytokinin plant growth regulator, has been banned for use in bean sprout production in China. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with a specific monoclonal antibody (mAb 3E5). The assay showed a half-maximum inhibition concentration (IC50) and detection range of 18.9 ng/mL and 3.6-106 ng/mL, respectively. Recoveries of 6-BA spiked in home cultured bean sprout samples averaged from 75% to 89% with a correlation coefficient (R2) of 0.998 between the results determined by icELISA and those by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS). LC-ESI-MS showed that 6-BA had been partially metabolized to 6-benzylaminopurine riboside (6-BAR) in the positive samples. The content of 6-BA determined by icELISA was about 5-70 times higher than that of LC-ESI-MS because mAb 3E5 had 315% cross-reactivity with 6-BAR. Such icELISA being ultra-sensitive to 6-BAR would allow quick monitoring of 6-BA by detecting 6-BAR as a potential biomarker. [Description provided by NIOSH]
• Subjects:
  Agriculture Antibodies Antigens Biological Assay Biomarkers Cell Division Energy Metabolism Enzymes Laboratories Occupational Health Purines Safety Safety Management

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• Keywords:
  Amino Acids Analytical Methods Author Keywords: 6-Benzylaminopurine Bioassays Cell Division Chemical Agent Detectors ELISA Enzyme-linked Immunosorbent Assay Food Food Safety Foodstuff Laboratory Techniques Metabolites Monoclonal Antibody Plant Growth Regulator Plants Plant Substances Safety Monitoring

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• ISSN:
  0308-8146
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Hawaii ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  233-238
• Volume:
  207
• NIOSHTIC Number:
  nn:20049808
• Citation:
  Food Chem 2016 Sep; 207:233-238
• Contact Point Address:
  Guiyu Tan, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
• Email:
  tanguiyu@cau.edu.cn
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a497e00c41a347fddae40c96e082b8de469ba70a55ca6eb9e4dbc075160603f7d267da06ece5a0e790bc752f6acf2a76e5ec07ef76489c2fd228ffd7cfd0fb3e
• Download URL:
  https://stacks.cdc.gov/view/cdc/210433/cdc_210433_DS1.pdf
• File Type:
  [PDF - 1.54 MB ]