Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Li, Linlin ; Diab, Santiago ; McGraw, Sabrina ; Barr, Bradd ; Traslavina, Ryan ; ... More +
Li, Linlin ; Diab, Santiago ; McGraw, Sabrina ; Barr, Bradd ; Traslavina, Ryan ; Higgins, Robert ; Talbot, Tom ; Blanchard, Pat ; Rimoldi, Guillermo ; Fahsbender, Elizabeth ; Page, Brady ; Phan, Tung Gia ; Wang, Chunlin ; Deng, Xutao ; Pesavento, Patricia ; Delwart, Eric Less -
Description:
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.
Subjects:
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Animals Astroviridae Astroviridae Infections Astrovirus Bovine Brain Cattle Cattle Diseases Expedited Genes, Viral In Situ Hybridization Mamastrovirus Metagenomics Molecular Sequence Data Nervous System Diseases Neurologic Disease Next-generation Sequencing Open Reading Frames Phylogeny Research Retrospective Studies Spinal Cord Viruses
Source:
Emerg Infect Dis. 19(9):1385-1392.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:7b7bf8c34b91c324feec01b6b3f3a106b8bb89f7c39a77de3b67385f037a7d45
Download URL:
https://stacks.cdc.gov/view/cdc/20908/cdc_20908_DS1.pdf
File Type:

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