Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Li, Linlin ; Diab, Santiago ; McGraw, Sabrina ; Barr, Bradd ; Traslavina, Ryan ; ... More +
Li, Linlin ; Diab, Santiago ; McGraw, Sabrina ; Barr, Bradd ; Traslavina, Ryan ; Higgins, Robert ; Talbot, Tom ; Blanchard, Pat ; Rimoldi, Guillermo ; Fahsbender, Elizabeth ; Page, Brady ; Phan, Tung Gia ; Wang, Chunlin ; Deng, Xutao ; Pesavento, Patricia ; Delwart, Eric Less -
Description:
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.
Subject:
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Animals
Astroviridae
Astroviridae Infections
Astrovirus
Bovine
Brain
Cattle
Cattle Diseases
Expedited
Genes, Viral
In Situ Hybridization
Mamastrovirus
Metagenomics
Molecular Sequence Data
Nervous System Diseases
Neurologic Disease
Next-generation Sequencing
Open Reading Frames
Phylogeny
Research
Retrospective Studies
Spinal Cord
Viruses
Brain
Cattle

Source:
Emerg Infect Dis. 19(9):1385-1392.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:7b7bf8c34b91c324feec01b6b3f3a106b8bb89f7c39a77de3b67385f037a7d45
File Type:

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