Rapid and Sensitive Detection of Wood Smoke Exposure Biomarkers Using Europium Fluorescent Nanoparticle Label/Lateral Flow Immunoassay

Details

• Personal Author:
  Du D ; Fu Y ; Lin Y ; Ruan X ; Song Y ; Yang Z
• Description:
  Exposure to wood smoke is associated with various adverse health problems. Biomonitoring of smoke exposure-associated biomarkers provides accurate measurements of personally absorbed doses. As a specific metabolite of benzene, the quantitative measurement of S-phenylmercapturic acid (S-PMA) plays a vital role in evaluating human exposure to wood smoke. In this study, we developed an efficient lateral flow immunoassay (LFIA) approach for accurately and rapidly measuring S-PMA levels. Europium chelate nanoparticles (EuNPs) conjugated with purified polyclonal sheep anti-S-PMA antibodies were employed as the fluorescent detection probe. This work is based on a competitive immunoassay, where the target S-PMA competes with the immobilized antigen on the test lines for the limited antigen-binding sites on EuNP-conjugated antibodies. Due to this competition, the fluorescent intensity of the EuNPs is inversely proportional to the concentration of the target S-PMA in the sample, enabling quantitative measurement. Owing to the large Stokes shift, superior fluorescent brightness, and saturation of the EuNPs, S-PMA levels can be measured with a limit of detection of 0.32 ng/mL, a detectable range of 0.10-30 ng/mL, and a linear detection range of 0.25-30 ng/mL under optimized conditions. Stability testing revealed that the LFIA strips can be stored at room temperature for up to one year while maintaining excellent detection performance for S-PMA. These results demonstrate that the EuNP-based LFIA is a promising tool for accurate preclinical and point-of-care evaluation of wood smoke exposure. A major advantage of this approach is its ability to accurately analyze smoke biomarkers at anticipated low concentrations. The sensor system allows low-cost, rapid, and on-site data collection and quantification of wood smoke exposure. [Description provided by NIOSH]
• Subjects:
  Biomarkers Fires Occupational Health Safety Wildfires Wood
• Keywords:
  Author Keywords: Lateral Flow Immunoassay Biomonitoring Europium Chelates Nanoparticles Immunoassays Nanoparticles S-phenylmercapturic Acid Smoke Inhalation Wildland Fire Smoke Wood Smoke Biomarker
• ISSN:
  0039-9140
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  San Francisco Region [OSHA] ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  291
• NIOSHTIC Number:
  nn:20070713
• Citation:
  Talanta 2025 Aug; 291:127760
• Contact Point Address:
  Dan Du, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164
• Email:
  annie.du@wsu.edu
• Federal Fiscal Year:
  2025
• NORA Priority Area:
  Public Safety
• Performing Organization:
  Washington State University
• Peer Reviewed:
  True
• Start Date:
  20230901
• Source Full Name:
  Talanta
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7fc781ac226adfabed89a65dee7c08173c699fb49cf3d976de44e8f407c0461ef5a02096db49ceb62a35cbcda27c0f76994f234352bb279604ddd45647a7b26e
• Download URL:
  https://stacks.cdc.gov/view/cdc/208885/cdc_208885_DS1.pdf
• File Type:
  [PDF - 4.91 MB ]