Toxicity Assessments of Selected Trichloroethylene and Perchloroethylene Metabolites in Three In Vitro Human Placental Models

Details

• Personal Author:
  Armant DR ; Bakulski KM ; Elkin ER ; Kilburn BA ; Loch-Caruso R ; Su AL
• Description:
  Residential and occupational exposures to the industrial solvents perchloroethylene (PERC) and trichloroethylene (TCE) present public health concerns. In humans, maternal PERC and TCE exposures can be associated with adverse birth outcomes. Because PERC and TCE are biotransformed to toxic metabolites and placental dysfunction can contribute to adverse birth outcomes, the present study compared the toxicity of key PERC and TCE metabolites in three in vitro human placenta models. We measured cell viability and caspase 3 + 7 activity in the HTR-8/SVneo and BeWo cell lines, and caspase 3 + 7 activity in first trimester villous explant cultures. Cultures were exposed for 24 h to 5-100 µM S-(1,2-dichlorovinyl)-L-cysteine (DCVC) and S-(1,2,2-trichlorovinyl)-L-cysteine (TCVC), or 5-200 µM trichloroacetate (TCA) and dichloroacetate (DCA). DCVC significantly reduced cell viability and increased caspase 3 + 7 activity in HTR-8/SVneo cells at a lower concentration (20 µM) compared with concentrations toxic to BeWo cells and villous explants. Similarly, TCVC reduced cell viability and increased caspase 3 + 7 activity in HTR-8/SVneo cells but not in BeWo cells. TCA and DCA had only negligible effects on HTR-8/SVneo or BeWo cells. This study advances understanding of potential risks of PERC and TCE exposure during pregnancy by identifying metabolites toxic in placental cells and tissues. [Description provided by NIOSH]
• Subjects:
  Occupational Health Pregnancy Safety Solvents Tetrachloroethylene Toxicology Urogenital System
• Keywords:
  Author Keywords: Trichloroethylene DCA DCVC Dichloroacetate HTR-8/SVneo In Vitro Studies PERC Perchloroethylene Prenatal Exposure Reproductive Effects S-(1,2,2-trichlorovinyl)-L-cysteine TCA TCE TCVC TetrachloroethyleneS-(1,2-dichlorovinyl)-L-cysteine Trichloroacetate Trichloroethylene

  More +
• ISSN:
  0890-6238
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  109-120
• Volume:
  109
• NIOSHTIC Number:
  nn:20070621
• Citation:
  Reprod Toxicol 2022 Apr; 109:109-120
• Contact Point Address:
  Elana R. Elkin, Department of Environmental Health Sciences, University of Michigan, 1415 Washington Heights, Ann Arbor, MI 48109, USA
• Email:
  elkinela@umich.edu
• CAS Registry Number:
  Hexachloroethane (CAS RN 67-72-1) ; Trichloroethylene (CAS RN 79-01-6)
• Federal Fiscal Year:
  2022
• Performing Organization:
  University of Michigan, Ann Arbor
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Reproductive Toxicology
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4cd26bc2068a1d3acc91714e36029b2876f7075481eb6756b41d01d0e2084b64af4dbcc604f3b7ab9590b550bfed503b6522ca28cddc7e2f0ab772a1469dcb0a
• Download URL:
  https://stacks.cdc.gov/view/cdc/208800/cdc_208800_DS1.pdf
• File Type:
  [PDF - 5.07 MB ]