Assessment of Dermal Sensitization by Nickel Salts in a Novel Humanized TLR-4 Mouse Model (Dataset)

Details

• Personal Author:
  Aldinger, Jeffrey A. ; Anderson S ; Roach K ; Roberts J ; Stefaniak, Aleksandr B. ; Waggy C
• Description:
  Nickel is one of the most common contact allergens worldwide. Despite this prevalence, many of the underlying immunological mechanisms responsible for nickel allergy remain unclear. This knowledge gap is partially attributable to the inherent resistance of laboratory rodents (mice and rats) to dermal sensitization with nickel salts. The fundamental goal of this study was to assess the potential utility of the novel humanized Toll-like receptor-4 (hTLR-4) mouse model for use in future in vivo studies of nickel allergy. Accordingly, hTLR-4 positive and negative mice of both sexes were first incorporated into a Local Lymph Node Assay (LLNA) to evaluate dermal sensitization following topical exposure to soluble nickel salts (NiSO4). The ensuing immune responses were then characterized further by incorporating female and male hTLR-4 positive mice into a non-radioactive endpoint-based assay. Utilizing the same exposure scheme as in the LLNA, mice were euthanized following exposure and the auricular lymph nodes, spleen, and blood were collected to assess various immunological parameters associated with allergy and ACD. [Description provided by NIOSH]
• Subjects:
  Animals Hypersensitivity Nickel Occupational Health Safety Skin
• Keywords:
  Allergic Reactions Allergies Animal Studies Dermal Exposures Sensitization Skin Sensitivity
• DOI:
  https://doi.org/10.26616/NIOSHRD-1103-2024-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  Philadelphia Region [OSHA] ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20070384
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1103-2024-0, 2024 Dec; :dataset
• Contact Point Address:
  Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1000 Frederick Lane (Mailstop 4020), Morgantown, WV 26508. Tel 304.285.6024
• CAS Registry Number:
  Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2025
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Assessment of dermal sensitization by nickel salts in a novel humanized TLR-4 mouse model
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a3ae6786c4ea745996c895d22711249b6f85db17ecd41261b17fc0ba816deddb005795d9c9b56a4f73b097a87fd3dbe00e89a1451cc4b14b3239851bf01496b2
• Download URL:
  https://stacks.cdc.gov/view/cdc/208614/cdc_208614_DS1.pdf
• File Type:
  [PDF - 100.51 KB ]