Zinc Protects Against Swine Barn Dust-Induced Cilia Slowing

Details

• Personal Author:
  Bauer CD ; Knoell DL ; Mosley DD ; Poole JA ; Samuelson DR ; Smith DR ; Wyatt TA
• Description:
  Agricultural workers exposed to organic dust from swine concentrated animal feeding operations (CAFOs) have increased chances of contracting chronic lung disease. Mucociliary clearance represents a first line of defense against inhaled dusts, but organic dust extracts (ODEs) from swine barns cause cilia slowing, leading to decreased bacterial clearance and increased lung inflammation. Because nutritional zinc deficiency is associated with chronic lung disease, we examined the role of zinc supplementation in ODE-mediated cilia slowing. Ciliated mouse tracheal epithelial cells were pretreated with 0-10 µg/mL ZinProTM for 1 h, followed by treatment with 5% ODE for 24 h. Cilia beat frequency (CBF) and protein kinase C epsilon (PKCe) activity were assayed. ODE treatment resulted in cilia slowing after 24 h, which was reversed with 0.5 and 1.0 µg/mL ZinPro pre-treatment. No zinc protection was observed at 50 ng/mL, and ciliated cells detached at high concentrations (100 µg/mL). ZinPro alone produced no changes in the baseline CBF and showed no toxicity to the cells at concentrations of up to 10 µg/mL. Pre-treatment with ZinPro inhibited ODE-stimulated PKCe activation in a dose-dependent manner. Based on ZinPro's superior cell permeability compared to zinc salts, it may be therapeutically more effective at reversing ODE-mediated cilia slowing through a PKCe pathway. These data demonstrate that zinc supplementation may support the mucociliary transport apparatus in the protection of CAFO workers against dust-mediated chronic lung disease. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Dust Environmental Exposure Lung Diseases Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Agricultural Author Keywords: Organic Dust Cilia Environmental Exposure Lung Lung Disease Organic Dusts Zinc
• ISSN:
  2218-273X
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  7
• NIOSHTIC Number:
  nn:20069943
• Citation:
  Biomolecules 2024 Jul; 14(7):843
• Contact Point Address:
  Todd A. Wyatt, Department of Internal Medicine, Division of Pulmonary, Critical Care & Sleep Medicine, University of Nebraska Medical Center, 985910 Nebraska Medical Center, Omaha, NE 68198
• Email:
  twyatt@unmc.edu
• CAS Registry Number:
  Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Biomolecules
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:128ddee0c49b5838c50621d2c2cd2ce750474530774bdbdb2498f530d057b3742a589e10e6593015908ee3eda8b1a1d8a4f47fc4516e70efe75d1981b1d74600
• Download URL:
  https://stacks.cdc.gov/view/cdc/208262/cdc_208262_DS1.pdf
• File Type:
  [PDF - 1.49 MB ]