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Surface Reaction of Electroosmotic Flow-Driven Free Antigens with Immobilized Magnetic-Microbeads-Tagged-Antibodies in Microchannels



Details

  • Personal Author:
  • Description:
    Immunoassays based on reactions between target pathogen (antigen; Ag) and antibody (Ab) are frequently used for Ag detection. An external magnetic field was used to immobilize magnetic microbeads-tagged-antibodies (mMB-Ab) on the surface of a microchannel in the capture zone. The mMB-Ab was subsequently used for Ag detection. The objective of this numerical study, with experimental validation, is to assess the surface reaction between mMB-Ab and Ag in the presence of electro-osmotic flow (EOF). First, immobilization of mMB-Ab complex in the wall of the capture zone was achieved. Subsequently, the Ag was transported by EOF toward the capture zone to bind with the immobilized mMB-Ab. Lastly, mMB-Ab:Ag complex was formed and immobilized in the capture zone. A finite volume solver was used to implement the above steps. The surface reaction between the mMB-Ab and Ag was investigated in the presence of electric fields (E): 150 V/cm-450 V/cm and Ag concentrations: 0.001 M-1000 M. The depletion of mMB-Ab increases with time as the E decreases. Furthermore, as the concentration of Ag decreases, the depletion of mMB-Ab increases with time. These results quantify the detection of Ag using the EOF device; thus, signifying its potential for rapid throughput screening of Ag. This platform technology can lead to the development of portable devices for the detection of target cells, pathogens, and biomolecules for testing water systems, biological fluids, and biochemicals. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0148-0731
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    146
  • Issue:
    9
  • NIOSHTIC Number:
    nn:20069535
  • Citation:
    J Biomech Eng 2024 Sep; 146(9):091003
  • Contact Point Address:
    Rupak K. Banerjee, Department of Biomedical Engineering, University of Cincinnati, Veterans Affairs Medical Center, Rhodes Hall 593, 2851 Woodside Drive, Cincinnati, OH 45219
  • Email:
    banerjr@ucmail.uc.edu
  • Federal Fiscal Year:
    2024
  • Performing Organization:
    University of Cincinnati
  • Peer Reviewed:
    True
  • Start Date:
    20050701
  • Source Full Name:
    Journal of Biomechanical Engineering
  • End Date:
    20260630
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:c699f5b73270b8bacbdd614d74c4ef7b594234574e801e659fe39303f74b9a38ea732d5f6bba18b3be454e9ab062fe94fbad68d842559185be13ff5b348bc619
  • Download URL:
  • File Type:
    Filetype[PDF - 2.86 MB ]
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