Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure (Dataset)

Details

• Personal Author:
  Anderson, Stacey E. ; Barnes MA ; Beezhold, Donald H. ; Blackwood CB ; Croston TL ; Germolec DR ; Goldsmith WT ; Green, Brett J. ; Lemons, Angela R. ; McKinney W ; Park, Ju-Hyeong ; Rush RE ; Sulyok M ; Weaver KL
• Description:
  Aspergillus versicolor is ubiquitous in the environment and is particularly abundant in damp indoor spaces. Exposure to Aspergillus species, as well as other environmental fungi, has been linked to adverse health outcomes including asthma, allergy, and even local or disseminated infection. However, the pulmonary immunological mechanisms associated with repeated exposure to A. versicolor have remained relatively uncharacterized. Here, A. versicolor was cultured and desiccated on rice, then placed in an acoustical generator system to achieve aerosolization. Mice were challenged with titrated doses of aerosolized conidia to examine deposition, lymphoproliferative properties, and then the immunotoxicological response to repeated inhalation exposures. The necessary dose to induce lymphoproliferation, but not infection-like pathology, was identified. Further, it was determined that the dose was able to initiate localized immune responses. The data presented in this study demonstrate an optimized and reproducible method for delivering A. versicolor conidia to rodents via nose-only inhalation. Additionally, the feasibility of a long-term repeated exposure study was established. This experimental protocol can be used in future studies to investigate physiological effects of repeated pulmonary exposure to fungal conidia utilizing a practical and relevant mode of delivery. In total, these data constitute an important foundation for subsequent research in the field. [Description provided by NIOSH]
• Subjects:
  Animals Fungi Immune System Occupational Health Respiratory System Safety
• Keywords:
  Animal Studies Aspergillus Infection Bioaerosols Histopathology Immune Reaction Immunological Tests Indoor Environments Inhalation
• DOI:
  https://doi.org/10.26616/NIOSHRD-1078-2023-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  Indiana ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20068892
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1078-2023-0, 2024 Jan; :dataset
• Contact Point Address:
  Allergy and Clinical Immunology Branch (ACIB), Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV
• Federal Fiscal Year:
  2024
• Peer Reviewed:
  False
• Source Full Name:
  Optimization of Aspergillus versicolor culture and aerosolization in a murine model of inhalational fungal exposure
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3861aa5c3f741c5084b29fcdd649004ddccb2ddf96f2c40b20ecbb235b1c24b70bc6668f62bb5891549d52334ff1a6702f21436c52988a9d75f328a36ce5a2c6
• Download URL:
  https://stacks.cdc.gov/view/cdc/207456/cdc_207456_DS1.pdf
• File Type:
  [PDF - 169.58 KB ]