Compositional Variations in Metal Nanoparticle Components of Welding Fumes Impact Lung Epithelial Cell Toxicity

Details

• Personal Author:
  Biggs K ; Lee CG ; Liao J ; Park JH ; Shannahan JH ; Xia L
• Description:
  Welding fumes contain harmful metals and gas by-products associated with development of lung dysfunction, asthma, bronchitis, and lung cancer. Two prominent welding fume particulate metal components are nanosized iron (Fe) and manganese (Mn) which might induce oxidative stress and inflammation resulting in pulmonary injury. Welding fume toxicity may be dependent upon metal nanoparticle (NP) components. To examine toxicity of welding fume NP components, a system was constructed for controlled and continuous NP generation from commercial welding and customized electrodes with varying proportions of Fe and Mn. Aerosols generated consisted of nanosized particles and were compositionally consistent with each electrode. Human alveolar lung A459 epithelial cells were exposed to freshly generated metal NP mixtures at a target concentration of 100?µg/m3 for 6?hr and then harvested for assessment of cytotoxicity, generation of reactive oxygen species (ROS), and alterations in the expression of genes and proteins involved in metal regulation, inflammatory responses, and oxidative stress. Aerosol exposures decreased cell viability and induced increased ROS production. Assessment of gene expression demonstrated variable up-regulation in cellular mechanisms related to metal transport and storage, inflammation, and oxidative stress based upon aerosol composition. Specifically, interleukin-8 (IL-8) demonstrated the most robust changes in both transcriptional and protein levels after exposure. Interleukin-8 has been determined to serve as a primary cytokine mediating inflammatory responses induced by welding fume exposures in alveolar epithelial cells. Overall, this study demonstrated variations in cellular responses to metal NP mixtures suggesting compositional variations in NP content within welding fumes may influence inhalation toxicity. [Description provided by NIOSH]
• Subjects:
  Aerosols Cytotoxins Lung Occupational Health Particulate Matter Respiratory System Safety Welding
• Keywords:
  Air-liquid Interface Exposure Alveolar Cells Author Keywords: Welding Fumes Composition Cytokines Cytotoxicity Inflammation Inhalation Metal Oxide Metal Oxides Metal Storage Metal Transport Nanoparticles Nanoparticles Mixtures Oxidative Stress Particulates Welding Fumes

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• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  86
• Issue:
  20
• NIOSHTIC Number:
  nn:20068535
• Citation:
  J Toxicol Environ Health A 2023 Oct; 86(20):735-757
• Contact Point Address:
  Jonathan H. Shannahan, School of Health Sciences, College of Health and Human Sciences, Purdue University, West Lafayette, IN, USA
• Email:
  jshannah@purdue.edu
• Federal Fiscal Year:
  2024
• Performing Organization:
  University of Michigan, Ann Arbor
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9fd4bb742ebae5b308c211b740ed36573abab463caa4c3e5420757bf4d6ffdb1c98c1f3eeb721b01c3e1dfed535d0e9621cb0b7466992016630b58e04cfb850a
• Download URL:
  https://stacks.cdc.gov/view/cdc/207146/cdc_207146_DS1.pdf
• File Type:
  [PDF - 4.76 MB ]