Uncoupling of oxidative phosphorylation in rat liver mitochondria by chloroethanols

Details

• Personal Author:
  Ansari GK ; Bhat HK
• Description:
  The effects of 2-chloroethanol (107073) (CE), 2,2-dichloroethanol (598389) (DCE) and 2,2,2-trichloroethanol (115208) (TCE) on the stimulation of mitochondrial respiration were compared, and a structure activity relationship was explored. Rat liver mitochondria were prepared from 2 month old male Sprague-Dawley- rats. With succinate as the respiratory substrate and using chloroethanols at 150 nanomolar, CE stimulated respiration by 28.2% and DCE by 202% while TCE inhibited mitochondrial respiration by more than 95%. The effect of change in the concentration of chloroethanols on mitochondrial respiration was also examined. CE showed maximum stimulation at 600 millimolar (mM), DCE at 150mM, and TCE at 30mM, 313%. Respiratory stimulation was not dependent upon mitochondrial protein concentration. Chloroethanols inhibited mitochondrial respiration when glutamate/malate was used as the respiratory substrate. Estimation of ATP showed that chloroethanols inhibited the synthesis of ATP. These findings indicated that chloroethanols stimulate mitochondrial respiration by uncoupling oxidative phosphorylation and that the uncoupling potency is proportional to the extent of chlorination at the beta position of haloethanol. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Hydrocarbons Laboratories Occupational Health Safety
• Keywords:
  Alcohols Author Keywords: Chloroethanols Chlorinated-hydrocarbons Cytotoxic-effects Grants-other In-vitro-studies Laboratory-animals Liver-microsomes Liver Mitochondria NIOSH-Grant NIOSH-Publication Uncoupling Of Oxidative Phosphorylation

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  203-211
• Volume:
  59
• Issue:
  1
• NIOSHTIC Number:
  nn:00204741
• Citation:
  Toxicol Lett 1991 Dec; 59(1-3):203-211
• Contact Point Address:
  Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
• CAS Registry Number:
  2,2-Dichloroethanol (CAS RN 598-38-9) ; 2-Chloroethanol (CAS RN 107-07-3) ; Trichloroethanol (CAS RN 115-20-8)
• Federal Fiscal Year:
  1992
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Texas Medical Branch, Galveston, Texas
• Peer Reviewed:
  True
• Start Date:
  19860101
• Source Full Name:
  Toxicology Letters
• End Date:
  19960630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5e8d9bfa9bd6129ee103305b4b97328078b24266ccb8ef233fdde21cd7494dcf0c7f198b567d71a9078a93814a2bad67e9e1f7d0c341bafe17fa4431def1d63b
• Download URL:
  https://stacks.cdc.gov/view/cdc/205821/cdc_205821_DS1.pdf
• File Type:
  [PDF - 619.41 KB ]