Taurine uptake by isolated alveolar macrophages and type II cells

Details

• Personal Author:
  Banks MA ; Castranova, Vincent ; Martin WG ; Pailes WH
• Description:
  The possibility that alveolar macrophages and type-II cells can accumulate taurine was examined. The study involved measuring the taurine content of the cells, the rates of taurine uptake in the presence of a normal plasma concentration of taurine, the concentration dependence and kinetic parameters for the transport system, and the effects of metabolic inhibitors or ouabain on the transport processes. Lung cells were isolated from male Sprague- Dawley-rats. Taurine uptake displayed two components: the initial component was rapid and of short duration, less than 30 minutes, whereas the second component of uptake was less rapid and of longer duration, 30 to 120 minutes. The rates of taurine influx during both phases of uptake were greater for alveolar macrophages than for type-II cells, although alveolar type-II cells had a higher taurine content than alveolar macrophages. Alveolar macrophages displayed a higher proportion of leakage than type-II cells. Thus the higher taurine content of alveolar type-II cells may be due both to differences in the surface area of the cells and to differences in the ratio of carrier mediated taurine uptake to leakage of taurine from the cells. [Description provided by NIOSH]
• Subjects:
  Amino Acids Energy Metabolism Hematologic Tests Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Amino-acids Blood-analysis Cellular-uptake In-vitro-studies Laboratory-animals Metabolic-study NIOSH-Author
• ISSN:
  8750-7587
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  66
• Issue:
  3
• NIOSHTIC Number:
  nn:00205590
• Citation:
  J Appl Physiol 1989; 66(3):1079-1086
• Federal Fiscal Year:
  1989
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Applied Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:238636e0993b96adf5db2893a87ccb7bccde096ac87397abad2afa43986220803b3abcca96b16a921c31c18ed2c557863ac0cb0c0a9b1c657e9872fac5f9aeba
• Download URL:
  https://stacks.cdc.gov/view/cdc/205675/cdc_205675_DS1.pdf
• File Type:
  [PDF - 1.75 MB ]