Taurine protects against oxidant injury to rat alveolar pneumocytes

Details

• Personal Author:
  Banks MA ; Castranova, Vincent ; Martin WG ; Porter DW ; Banks MA ; Castranova, Vincent ; Martin WG ; Porter DW
• Description:
  The ability of the amino acid taurine to protect against ozone (10028156) induced oxidative injury was studied in rat alveolar macrophages. Alveolar macrophages obtained from male Sprague-Dawley- rats were exposed to 0 or 0.45 part per million ozone for up to 60 minutes (min) following pretreatment with 100 to 500 micromolar (microM) taurine added to the extracellular medium. The effects on cellular viability, resting and zymosan stimulated chemiluminescence used as a marker for reactive oxygen species generation, total ATPase and sodium, potassium dependent ATPase (Na,KATPase) activity, leakage of lipid peroxides, reduced glutathione (GSH), protein, and potassium ion (K+) into the extracellular medium, and intracellular and extracellular taurine concentrations were determined. Ozone exposure significantly decreased macrophage viability, resting and zymosan stimulated chemiluminescence, and total ATPase and Na,KATPase activity in a time dependent manner. Leakage of lipid peroxides, protein, GSH, and K+ into the medium was significantly increased in a time dependent manner. Intracellular taurine concentrations increased with increasing length of ozone exposure for up to 30min and then decreased thereafter. Extracellular taurine concentrations increased progressively with increasing length of ozone exposure. Adding taurine to the extracellular medium resulted in increasing intracellular taurine concentrations. Taurine enrichment countered the effects of ozone on viability, lipid peroxidation, total ATPase activity, and GSH leakage in a dose dependent manner. Taurine at 100microM significantly countered the effects on Na,KATPase activity and protein leakage. The authors conclude that taurine protects against ozone induced injury in rat alveolar macrophages. The protective effect of taurine is thought to reflect its being mobilized from intracellular stores to the free state in response to ozone exposure. [Description provided by NIOSH]
• Subjects:
  Amino Acids Antioxidants Cytotoxins Irritants Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Amino-acids Cellular-function Cytotoxic-effects Dose-response In-vitro-studies Irritant-gases Lipid-peroxidation Mammalian-cells Prophylaxis
• ISBN:
  9780306442247
• Publisher:
  Plenum Press
• Document Type:
  Text
• Genre:
  Proceedings ; Proceedings
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies ; DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  341-354
• NIOSHTIC Number:
  nn:00232437
• Citation:
  Taurine: nutritional value and mechanisms of action - proceedings of the Waltham Symposium on taurine and cat nutrition, October 8, 1991, the International Taurine Symposium: new dimensions on Its mechanisms of action, October 9-10, 1991, Orange Beach, Alabama. Lombardini JB, Schaffer SW, Azuma J, eds. New York: Plenum Press, 1992:341-354
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6) ; Ozone (CAS RN 10028-15-6)
• Editor(s):
  Lombardini JB; Schaffer SW; Azuma J ; Lombardini JB; Schaffer SW; Azuma J
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  False
• Source Full Name:
  Taurine: nutritional value and mechanisms of action - proceedings of the Waltham Symposium on taurine and cat nutrition, October 8, 1991, the International Taurine Symposium: new dimensions on Its mechanisms of action, October 9-10, 1991, Orange Beach, Alabama
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:285110354876dff263406949123cd0c67a24e37e8cce0d90fbffc086edc3c86c947db30b1d6bf67273bc83575dc4ecc9327d1ebad242a8563b8527e81d343527
• Download URL:
  https://stacks.cdc.gov/view/cdc/205674/cdc_205674_DS1.pdf
• File Type:
  [PDF - 1.27 MB ]