Superoxide anion production in response to bacterial lipopolysaccharide and fungal spores implicated in organic dust toxic syndrome

Details

• Personal Author:
  Lewis, Daniel M. ; Shahan TA ; Sorenson WG
• Description:
  The effects of fungal spores and bacterial lipopolysaccharide (LPS) on superoxide anion production and guinea-pig bronchial alveolar lavage (BAL) cell activation was studied to determine the link between fungal spores, gram negative bacteria and their endotoxins to organic dust toxic syndrome (ODTS). BAL cells from English-Short- Hair-Guinea-pigs were incubated with LPS and/or fungal spores from Aspergillus-candidus, Aspergillus-terreus, Aspergillus-niger, Aspergillus-fumigatus, Eurotima-amstelodami, Penicillium-spinulosum and Cladosporium-cladosporioides, isolated from material associated with ODTS. Superoxide anion release was measured spectrophotometrically at a wavelength of 570 nanometers through the reduction of cytochrome-c using a 96 well plate reader. Spores from each fungus were able to effect an increase in superoxide anion production. The amount of production varied with each species. The study showed that LPS used alone did little to induce BAL cell activation. However, pretreating cells with LPS and then exposing them to fungal spores resulted in an increase in superoxide anion production which exceeded that of either the spores or LPS used separately. Thirty minutes was found to be the best pretreatment interval. The authors conclude that bacterial LPS strongly stimulates cell activation by fungal spores. [Description provided by NIOSH]
• Subjects:
  Dust Fungi Hazardous Substances Laboratories Lung Microbiology Occupational Health Respiratory System Safety
• Keywords:
  In-vitro-studies Laboratory-animals Lung-cells Microorganisms NIOSH-Author Organic-dusts Toxins
• ISSN:
  0013-9351
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  98-107
• Volume:
  67
• Issue:
  1
• NIOSHTIC Number:
  nn:00224596
• Citation:
  Environ Res 1994 Oct; 67(1):98-107
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ffed5d1e0e2326dfe6ed271d60eb6cafe6ec2dd4c77827e8f6c1eb64b7ea275974a821d90dd1d24adf4a30d72381225df06eac37f757ae3bb06b6ae1a80f1d27
• Download URL:
  https://stacks.cdc.gov/view/cdc/205650/cdc_205650_DS1.pdf
• File Type:
  [PDF - 481.70 KB ]