SEM studies on the in vivo uptake of Aspergillus terreus spores by alveolar macrophages.

Details

• Personal Author:
  Green FH ; Major, Pervis C. ; Olenchock, Stephen A. ; Willard PA
• Description:
  The uptake of Aspergillus-terreus (Aspergillus) spores by alveolar macrophages was studied in-vivo. Male New-Zealand-white-rabbits were intratracheally instilled with 7 x 10(6) Aspergillus spores in a volume of 1.0 milliliter (ml). CRL/BR-rats were instilled intratracheally with 5 x 10(7) Aspergillus spores in 0.25ml. The animals were killed at selected times up to 2 hours (rabbits) or 48 hours (rats) after treatment, and the lungs were removed and sectioned. The sections were examined by light and electron microscopy supplemented by heavy metal staining and back scattered electron imaging. Sera from all animals were examined for precipitating antibodies by counterimmunoelectrophoresis against commercially available Aspergillus extract. All animals were negative for demonstrable precipitating antibodies. Uptake of Aspergillus spores by macrophages on the surface of the alveoli and airways was extremely rapid. More than 95 percent of the spores were associated with macrophages 3 hours after treatment. The number of spores within a macrophage ranged from one to 20. No spores (either free or within macrophages) were seen penetrating the bronchial or alveolar epithelium at any time after exposure. Very few polymorphonuclear leukocytes were seen. Macrophages with intracytoplasmic spores were seen on the bronchial mucosa at all times up to 48 hours. Between 3 and 48 hours, the number of macrophages containing spores decreased rapidly in the peripheral alveoli, whereas the number of macrophages arising from the respiratory bronchioles increased. The authors conclude that in- vivo phagocytosis of spores is an extremely rapid event in rats and rabbits and only minimal transport of spores through the alveolar wall seems to occur. [Description provided by NIOSH]
• Subjects:
  Fungi Laboratories Lung Microbiology Occupational Health Respiratory System Safety
• Keywords:
  Lung-cells; Alveolar-cells; Absorption-rates; Fungi; Lung-tissue; In-vivo-studies; Laboratory-animals; Microorganisms; Microscopic-analysis; Phagocytes
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  307-314
• NIOSHTIC Number:
  nn:00168045
• Citation:
  Scanning Electron Microscopy, SEM, Inc., AMF O'Hare; Chicago, Illinois 1980:307-314
• Federal Fiscal Year:
  1980
• Peer Reviewed:
  False
• Source Full Name:
  Scanning Electron Microscopy, SEM, Inc., AMF O'Hare; Chicago, Illinois
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242223/cdc_242223_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]