Purification and characterization of cytochrome P450 isozymes from β/beta-naphthoflavone-induced adult hen liver

Details

• Personal Author:
  Abou-Donia MB ; Gupta RP ; Lapadula DM
• Description:
  Cytochrome-P450 isozymes induced in hens by a classic polycyclic aromatic hydrocarbon beta-naphthoflavone (6051872) were isolated and characterized. Adult leghorn-hens were injected intraperitoneally with 2% beta-naphthoflavone at 80mg/kg daily for 4 days; 24 hours after the last dose, hens were killed, and the liver microsomes were isolated. The isolation procedure was a modification of the method used for purifying cytochrome-P450 isozymes from phenobarbital (PB) induced hen livers. Cytochromes-P450 beta-NF-A1/beta-NF-A2 were identical or very similar to PB induced PB-A, as all of them exhibited almost the same amino terminal sequence, mobilities during one and two dimensional electrophoresis, and other properties. The other two immunochemically related cytochrome-P450s, beta-NF-B and beta-NF-C, were distinct isozymes with different N-terminal amino acid sequences, and several biophysical and biochemical properties. The amino terminal sequences of cytochrome-P450 beta-NF-B and beta- NF-C were also different from other cytochrome-P450s purified from mammalian or nonmammalian species. The authors conclude that purification of these P450s and availability of antiserums will be useful in examining their role in the metabolism of various environmental neurotoxic compounds. [Description provided by NIOSH]
• Subjects:
  Chemistry Enzymes Laboratories Nervous System Nervous System Diseases Occupational Health Safety
• Keywords:
  Chemical-structure Enzyme-activity Laboratory-animals Liver-microsomal-enzymes Nervous-system-disorders Neurotoxic-effects NIOSH-Grant NIOSH-Publication
• ISSN:
  0003-9861
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  170-182
• Volume:
  282
• Issue:
  1
• NIOSHTIC Number:
  nn:00214021
• Citation:
  Arch Biochem Biophys 1990 Oct; 282(1):170-182
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  β-Naphthoflavone (CAS RN 6051-87-2)
• Federal Fiscal Year:
  1991
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Archives of Biochemistry and Biophysics
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:175e825c9b3501224a118eb99ae143eb062c12eaff34f1434e986c7a7581298a29df6b442ba53715d07678fa958d98e44a00e946c5af5d86bbe7e6cdcfe4ce19
• Download URL:
  https://stacks.cdc.gov/view/cdc/205439/cdc_205439_DS1.pdf
• File Type:
  [PDF - 4.34 MB ]