Induction of hepatic microsomal cytochrome P-450 and inhibition of brain, liver, and plasma esterases by an acute dose of S,S,S-tri-n-butyl phosphorotrithioate (DEF) in the adult hen

Details

• Personal Author:
  Abou-Donia MB ; Carrington CD ; Lapadula DM
• Description:
  The effects of oral and dermal administration of S,S,S-tri-n-butyl- phosphorotrithioate (150505) (DEF) on specific enzymes in the nervous system and plasma were investigated in leghorn-hens. Hens were administered 100, 200, 500, or 1000mg/kg DEF dermally or orally; 24 hours later, hens were decapitated. To determine the effects of microsomal enzyme inducers, hens were treated with sodium- phenobarbital or 3-methylcholanthrene daily for 3 days, or were administered a single dermal dose of 1000mg/kg DEF. Hens administered dermal doses or large oral doses of 500 and 1000mg/kg DEF or parathion (negative control) exhibited signs of acute cholinergic toxicity within 24 hours of dosing. Hens treated with 500mg/kg tri-o-cresyl-phosphate (TOCP) as a positive control did not show acute poisoning signs. The findings demonstrate that inhibition of the three nonspecific esterases is associated with the DEF dose and the dermal route of entry that produces organophosphorus induced delayed neurotoxicity (OPIDN). Parathion and orally administered DEF did not cause OPIDN and did not inhibit these esterases to the same extent as did TOCP and dermally applied DEF, both of which are capable of producing OPIDN. [Description provided by NIOSH]
• Subjects:
  Agriculture Enzymes Herbicides Laboratories Nervous System Nervous System Diseases Occupational Health Organophosphates Safety
• Keywords:
  Agricultural-chemicals Enzyme-activity Laboratory-animals Nervous-system-disorders Neuropathology Neurotoxic-effects NIOSH-Grant NIOSH-Publication Organo-phosphorus-compounds
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  300-310
• Volume:
  73
• Issue:
  2
• NIOSHTIC Number:
  nn:00214019
• Citation:
  Toxicol Appl Pharmacol 1984 Apr; 73(2):300-310
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  Merphos (CAS RN 150-50-5)
• Federal Fiscal Year:
  1984
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fb1b426e5389dcb15b52530860143c2bd72b48ad5c44958dc348b0d7004a2a3b338e878eb9cac457c2c0cf2d98ac026556c2763d852d2bf9b842ac032d963a7e
• Download URL:
  https://stacks.cdc.gov/view/cdc/204917/cdc_204917_DS1.pdf
• File Type:
  [PDF - 661.93 KB ]