Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report

Details

• Personal Author:
  Chapin RE ; Clegg ED ; Dostal LA ; Foote RH ; Hurtt ME ; Klinefelter GR ; Makris SL ; Perreault SD ; Schrader, Steven M. ; Seed J ; Seyler D ; Sprando R ; Treinen KA ; Veeramachaneni DN ; Wise LD
• Description:
  In an effort to facilitate data sharing and interlaboratory interpretation of reproductive toxicity, studies involving sperm motility testing optimal methods for such testing in rats, rabbits, and dogs were discussed by a working group convened by the ILSI Risk Science Institute as part of its cooperative agreement with the US Environmental Protection Agency, Office of Pesticide Programs. The consensus report reached by the working group was described in this article. Based on past studies the working group concluded that sampling from either the vas deferens or from the cauda epididymis was adequate for studies of rat sperm motility. It was stressed that optimal sampling would, however, be dependent upon the age and sexual activity of the rat as well as on the particular question being addressed. The group also recommended the diffusion method rather than the aspiration method for sperm collection and limiting manipulations associated with shear stress. It was suggested that strict temperature control was not necessary but maintenance of temperatures within the range of room temperature to 37 degrees-C during tissue processing and sperm collection and from 34 to 37 degrees-C during analysis was recommended. Other recommendations included the use of any physiologic buffered saline solution at physiologic pH with the addition of 0.5 to 1% bovine serum albumin, the use of 40 to 50 micron deep sample chambers, and measurements of velocity as well as percent motility values. Recommendations for sample preparation for morphology, classification of morphology, and rat sperm counts, and for rabbits and dogs, were presented. Future directions in this field were discussed. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Analytical-methods Author Keywords: Methods Dog In-vitro-studies Laboratory-animals Laboratory-techniques NIOSH-Author Rabbit Rat Reproductive-effects Reproductive-hazards Spermatozoa Sperm Counts Sperm Morphology Sperm Motility

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• ISSN:
  0890-6238
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  237-244
• Volume:
  10
• Issue:
  3
• NIOSHTIC Number:
  nn:00230788
• Citation:
  Reprod Toxicol 1996 May; 10(3):237-244
• Contact Point Address:
  Dr. Jennifer Seed, ILSI Risk Science Institute, 1126 16th St. N.W., Washington, DC 20036
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  True
• Source Full Name:
  Reproductive Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:de66fe27b39a33bc78e867b5ec12d68ebd4f662c5d159e831cbc77e04c3e8799b1dc28e10a165628f58eb68563255189e38414a2e1615bb648770a176dce890a
• Download URL:
  https://stacks.cdc.gov/view/cdc/205104/cdc_205104_DS1.pdf
• File Type:
  [PDF - 1.07 MB ]