Semen analysis and fertility assessment in rabbits: statistical power and design considerations for toxicology studies

Details

• Personal Author:
  Chapin RE ; Gladen BC ; Phelps JL ; Schrader, Steven M. ; Turner TW ; Williams J
• Description:
  The within and between rabbit variability for various parameters analyzed in semen were determined. The endpoints chosen were based on their origin within the male reproductive tract and can therefore be considered as potential functional markers for such sites. An attempt was made to determine the minimum number of sperm required for normal fertility in the New-Zealand-white-rabbit. Sperm concentration and total number were among the most variable parameters analyzed in rabbit semen. The results demonstrated that collecting four ejaculates/male once a week was the least variable of the collection frequencies evaluated. The inclusion of a preexposure period into the study design increased the statistical power for all semen parameters. In assessing a toxic insult on spermatogenesis in the rabbit via semen analysis, a treatment/post treatment period of 9 to 10 weeks is the minimum time that should be considered. The overall variability of a study is comprised of biological and analytical variation, and the latter should be reduced as much as possible. The results showed that one million motile sperm can be considered the minimum number for normal reproductive capacity of the New-Zealand-white-rabbit. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Laboratories Occupational Health Safety Toxicology Urogenital Diseases Urogenital System
• Keywords:
  Comparative-toxicology Cytotoxic-effects Laboratory-animals NIOSH-Author Reproductive-effects Reproductive-hazards Reproductive-system Spermatozoa
• ISSN:
  0272-0590
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  15
• Issue:
  4
• NIOSHTIC Number:
  nn:00198642
• Citation:
  Fundam Appl Toxicol 1990 Nov; 15(4):651-665
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  Fundamental and Applied Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7220a2b4e258db9ff90c3b6d7029d09d10c4a18c99a06c3fb05c57c6f8a03768ab548b3fbf4b25710ea9010297be62ab86812fb0882f4586e00e02b52198e6c2
• Download URL:
  https://stacks.cdc.gov/view/cdc/205571/cdc_205571_DS1.pdf
• File Type:
  [PDF - 1.57 MB ]