Inhibition of metabolic coupling by metals

Details

• Personal Author:
  Corcos IA ; Loch-Caruso R ; Trosko JE
• Description:
  This study examined whether certain metal compounds could alter junctional communication. Metabolic coupling was used to assay cell to cell communication in cultured Chinese-hamster-V79 cells. Wild type Chinese-hamster-V79 cells proficient in metabolism of 6- thioguanine (6-TG) were cocultured with mutant V79 cells unable to metabolize 6-TG to its toxic metabolite (resistant cells). Inhibition of metabolic coupling in the presence of 6-TG by the metals was demonstrated as an increase in the recovery of 6-TG resistant cells. Significantly increased recoveries of 6-TG resistant cells were demonstrated by the toxic metal compounds arsenic(V)-acid (1327522), mercurous-chloride (7546307), lead(II)- acetate (15347576), and nickel(II)-chloride (7718549) at concentrations which did not change cell survival. Since the increased recovery with nickel showed no dependence on concentration, its significance may be negligible. Cadmium-chloride (10108642) increased 6-TG resistant cell recovery, but only at a toxic concentration. Zinc-sulfate (7733020) did not increase recovery at all. This cellular response to arsenic (7440382), mercury (7439976), and lead (7439921), the inhibition of junction mediated intercellular communication, has not been previously reported. [Description provided by NIOSH]
• Subjects:
  Arsenic Cadmium Cells, Cultured Cytotoxins Inorganic Chemicals Lead Lead Poisoning Mercury Metals Occupational Health Poisoning Safety Urogenital Diseases

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• Keywords:
  Arsenic-compounds Arsenic-poisoning Cadmium-compounds Cell-cultures Cytotoxic-effects Lead-compounds Lead-poisoning Mercury-compounds Mercury-poisoning Metal-poisoning NIOSH-Grant NIOSH-Publication Reproductive-system-disorders

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• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  33-48
• Volume:
  32
• Issue:
  1
• NIOSHTIC Number:
  nn:00197790
• Citation:
  J Toxicol Environ Health 1991 Jan; 32(1):33-48
• Contact Point Address:
  Environmental & Indust Health University of Michigan 109 Observatory Street Ann Arbor, MI 48109-2029
• CAS Registry Number:
  Arsenic (CAS RN 7440-38-2) ; Arsenic acid (H3AsO4) (CAS RN 1327-52-2) ; Cadmium chloride (CAS RN 10108-64-2) ; Lead (CAS RN 7439-92-1) ; Lead acetate (CAS RN 15347-57-6) ; Mercury (CAS RN 7439-97-6) ; Mercury chloride (HgCl) (CAS RN 7546-30-7) ; Nickel dichloride (CAS RN 7718-54-9) ; Zinc sulfate (CAS RN 7733-02-0)
• Federal Fiscal Year:
  1991
• NORA Priority Area:
  Reproductive System Disorders
• Performing Organization:
  University of Michigan at Ann Arbor, Ann Arbor, Michigan
• Peer Reviewed:
  True
• Start Date:
  19860201
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19870831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:14dd7db7d059700ea48b5e4c579cfab8105aeb91198c019e2ae5ad5d2869d8850d2102abed07235736c740018f2efaefe8e49ca3e7f987b5aa49eaa1456e20f8
• Download URL:
  https://stacks.cdc.gov/view/cdc/204954/cdc_204954_DS1.pdf
• File Type:
  [PDF - 654.02 KB ]