Mechanisms of Cytoskeletal Injury by Heavy Metals

Details

• Personal Author:
  Perrino BA
• Description:
  The major aim of this study was to determine whether metallothionein (MT) disassembly and inhibition of assembly caused by cadmium (7440439), mercury (7439976), and lead (7439921) in cultured 3T3 cells was due to binding to tubulin sulfhydryl groups or binding to and activation of CaM. Methylmercury was used as a model for adverse effects to MT due to tubulin sulfhydryl binding in the extracted cytoskeleton model system and during the polymerization of purified MT proteins in-vitro. The results indicated that, like calcium, the inhibitory effect of cadmium on the polymerization of purified MT proteins containing tubulin and microtubule associated proteins was enhanced by exogenously added CaM, suggesting that microtubule associated proteins were involved in mediating the sensitivity of MT to cadmium activated CaM. The findings that cadmium, like calcium, can support the binding of CaM to tubulin and MAPs in Western blots of purified MT proteins support the proposal that MAPs are important in the increased sensitivity of MT to cadmium activated CaM. The stimulation by cadmium or calcium/CaM dependent protein-kinase-II, resulting in MAP phosphorylation and MT disassembly and inhibition of assembly was not investigated. [Description provided by NIOSH]
• Subjects:
  Lead Lead Poisoning Mercury Metals Metals, Heavy Occupational Health Poisoning Safety
• Keywords:
  Grants-other Heavy-metal-poisoning Lead-poisoning Mercury-poisoning Metallic-poisoning NIOSH-Grant Protein-chemistry Protein-synthesis
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-4
• NIOSHTIC Number:
  nn:00192780
• NTIS Accession Number:
  PB90-163742
• Citation:
  Department of Microbiology, School of Medicine, Boston University, Boston, Massachusetts 1989 Jun; :1-4
• Contact Point Address:
  Microbiology Boston University 80 East Concord Street Boston, Mass 02118
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Lead (CAS RN 7439-92-1) ; Mercury (CAS RN 7439-97-6)
• Federal Fiscal Year:
  1989
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Boston University, Boston, Massachusetts
• Peer Reviewed:
  False
• Start Date:
  19860929
• Source Full Name:
  Department of Microbiology, School of Medicine, Boston University, Boston, Massachusetts
• End Date:
  19881130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:543c221508df5c00cacc44b3b5dde41be7016db7e897c1d1dd088d51c2988a7241e20ad518371b96c490881daeadaad4ca04cd8c0c4541b1d335ac86c9dec6e1
• Download URL:
  https://stacks.cdc.gov/view/cdc/217781/cdc_217781_DS1.pdf
• File Type:
  [PDF - 211.72 KB ]