Generation of oxygen radicals by minerals and its correlation to cytotoxicity

Details

• Personal Author:
  Vallyathan V
• Description:
  A study was conducted to evaluate the potential role of several occupational mineral dusts in the generation of hydroxyl radicals and to correlate in-vitro cytotoxicity and lipid peroxidation potential with oxygen radical generation. Electronic spin resonance was used to monitor the generation of hydroxyl radicals in suspensions of mineral fibers with hydrogen-peroxide. Cytotoxicity of the minerals was determined by hemolysis of red blood cells and the release of lactate-dehydrogenase in alveolar macrophages isolated from Sprague-Dawley-rats. Spectrophotometric methods were used to measure surface iron content in mineral samples. Lipid peroxidation was assessed by incubation of mineral fibers with linoleic-acid for 1 hour. The concentration of surface iron in minerals was shown to be directly related to the potential for hydroxyl radical generation, although hydroxyl radical generation by higher iron containing minerals did not appear directly related to the cytotoxicity of minerals or to their potential to induce lipid peroxidation. A better correlation was observed with lipid peroxidation and the known pathogenicity of dusts. The author concludes that neither in-vitro toxicity studies nor the ability of the minerals to generate the more potent oxidizing agents such as hydroxyl radicals from hydrogen-peroxide can directly predict the potential for toxicity and resultant pulmonary fibrosis. An apparently better correlation exists between the ability of the minerals to induce lipid peroxidation and known toxicity and fibrogenicity in humans. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Dust Enzymes Lung Occupational Health Pneumoconiosis Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Oxygen Radicals Cellular-reactions Cytotoxic-effects Cytotoxicity Enzymatic-effects Enzymes Free-radical-generation Hemolysis Lipid Peroxidation Lung-irritants Mineral-dusts NIOSH-Author Pneumoconiosis Respiratory-system-disorders Surface Iron

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• ISSN:
  0091-6765
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  111-115
• Volume:
  102
• NIOSHTIC Number:
  nn:00227393
• Citation:
  Environ Health Perspect 1994 Dec; 102(Suppl 10):111-115
• Federal Fiscal Year:
  1995
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Health Perspectives
• Supplement:
  10
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:90f83877fbb38307b91101d7801f02814f83e7380952fdc394d410f696a5c8583dd66f71d95c6a883bcdb209db8f9255e9cbfc1d333ac4bed9b530f4fa0d0968
• Download URL:
  https://stacks.cdc.gov/view/cdc/204807/cdc_204807_DS1.pdf
• File Type:
  [PDF - 458.20 KB ]