Differential effects of triphenylphosphite and diisopropyl phosphorofluoridate on catecholamine secretion from bovine adrenomedullary chromaffin cells

Details

• Personal Author:
  Abou-Donia MB ; Knoth-Anderson J
• Description:
  The neurotoxic effects of triphenyl-phosphite (115866) (TPP) were studied. Chromaffin cells were isolated from bovine adrenal medullae and exposed to TPP or diisopropyl-phosphorofluoridate (55914) (DFP) for 24 hours with or without preincubation with nicotine, veratridine or potassium. A time and dose dependent decrease in the release of catecholamines by the chromaffin cells was seen following incubation with TPP. An inhibition of nicotine, veratridine, and potassium ion induced secretion was seen as well with exposure to TPP. Nicotine induced secretion was not affected by incubation with DFP or paraoxon (311455); however, these compounds significantly increased secretion in the presence of potassium. Nicotine or potassium stimulated calcium uptake by the cells was inhibited by TPP and unaffected by DFP. Under basal conditions, however, TPP stimulated calcium uptake and DFP inhibited calcium uptake after 5 minutes of incubation. Both TPP and DFP inhibited the activity of the enzyme neurotoxic-esterase. The authors conclude that the mechanism of TPP induced toxicity may involve changes in cell secretory processes. [Description provided by NIOSH]
• Subjects:
  Biochemistry Nervous System Occupational Health Organophosphates Safety
• Keywords:
  Biochemical-analysis Cellular-function In-vitro-study Mammalian-cells Neurotoxic-effects Neurotransmitters NIOSH-Grant NIOSH-Publication Organo-phosphorus-compounds
• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  103-114
• Volume:
  38
• Issue:
  2
• NIOSHTIC Number:
  nn:00212980
• Citation:
  J Toxicol Environ Health 1993 Feb; 38(2):103-114
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  Diisopropyl fluorophosphate (CAS RN 55-91-4) ; Paraoxon (CAS RN 311-45-5) ; Triphenyl phosphate (CAS RN 115-86-6)
• Federal Fiscal Year:
  1993
• NORA Priority Area:
  Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:50ffd5cf8a2086500f9661d6252dc1f72f71cbd0b1fb36db796a0ddbcba7a5aeeace3f4179eb464b411a3d4324a5c4d12176352ca996f2c0fcf9f0200cdf30a8
• Download URL:
  https://stacks.cdc.gov/view/cdc/204493/cdc_204493_DS1.pdf
• File Type:
  [PDF - 768.73 KB ]