Chloroform-induced multiple forms of ornithine decarboxylase: differential sensitivity of forms to enhancement by diethyl maleate and inhibition by ODC-antizyme

Details

• Personal Author:
  Bedell C ; DeAngelo AB ; Nofzinger K ; Pereira MA ; Savage RE Jr.
• Description:
  The effect of pretreatment with the glutathione depleting agent diethyl-maleate on the chloroform (67663) induction of two different forms of male rat liver ornithine-decarboxylase (ODC) activity, and the sensitivity of the two forms of the enzyme to inhibition by ODC- antizyme (ODC-AZ) were studied in rats. Male Fischer-344-rats were given a 3 hour pretreatment with diethyl-maleate at 325mg/kg, intraperitoneally (ip), prior to chloroform administration at 6.0 nanomoles/kilogram, ip for 18 hours. Treatment of animals with chloroform alone resulted in stimulations of Peak-I and Peak-II activities to a 5.5 and 12.0 fold increase over controls, respectively. Pretreatment with diethyl-maleate followed by the same dose of chloroform resulted in a minor increase in Peak-I but a continued increase in the ODC activity associated with Peak-II of three fold over chloroform alone and 36 fold over controls. With chloroform alone, Peak-II activity constituted 35 percent of the total recoverable ODC activity from the cytoplasm, while pretreatment of animals with diethyl-maleate followed by chloroform resulted in Peak-II activities accounting for 70 percent of the total ODC recoverable activity. The Peak-II form of ODC isolated from male rat liver was slightly more sensitive to ODC-AZ inhibition than was the Peak-I form of the enzyme. [Description provided by NIOSH]
• Subjects:
  Digestive System Digestive System Diseases Enzymes Hazardous Substances Hydrocarbons Laboratories Liver Occupational Health Safety
• Keywords:
  Chlorinated-hydrocarbons Enzyme-activity Hepatotoxicity Laboratory-animals Liver-damage Liver-enzymes NIOSH-Author Toxic-effects
• ISSN:
  0098-4108
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  57-64
• Volume:
  27
• Issue:
  1
• NIOSHTIC Number:
  nn:00188804
• Citation:
  J Toxicol Environ Health 1989 May; 27(1):57-64
• CAS Registry Number:
  Chloroform (CAS RN 67-66-3)
• Federal Fiscal Year:
  1989
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bd4f0329e4dbae45a8e6a469a7c18f2c7bc229476f35718f65d63faf6e5789ae4020285e7b7e94dc3d787792c6ac6b315af83b72d922bc672d087d1cbff0cc02
• Download URL:
  https://stacks.cdc.gov/view/cdc/204323/cdc_204323_DS1.pdf
• File Type:
  [PDF - 556.00 KB ]