Analysis of n-hexane, 2-hexanone, 2,5-hexanedione, and related chemicals by capillary gas chromatography and high-performance liquid chromatography

Details

• Personal Author:
  Abou-Donia MB ; Nomeir AA
• Description:
  Analytical methods are described for the separation and quantification of n-hexane (110543) and 11 of its related chemicals in one chromatographic system using two capillary gas chromatography methods. In the first method, the temperature program called for isothermic at 50 degrees-C for 30 minutes, followed by a temperature increase of 10 degrees/minute to a final temperature of 180 degrees- C, which was maintained for 7 minutes. The second method called for the isothermic to be 70 degrees-C for 15 minutes, followed by a temperature increase of 40 degrees/minute to a final temperature of 220 degrees-C. This final temperature was maintained for 5 minutes. With the first method, a linear relationship between peak area and amount injected was observed over a range of 0.2 to 10 micrograms. The minimum detectable amounts were 0.05 microgram for n-hexane, cyclohexane (110827), and 2,5-dimethylfuran (625865) and 0.2 microgram for the other compounds tested. A linear relationship between peak area and amount injected was also observed with the second method. An analysis was also performed using a mobile phase which was a linear gradient of 3 to 55 percent 2-propanol in n- hexane in a period of 1 minute at a solvent delivery rate of 0.8 milliliters/minute. Compounds were detected by monitoring the ultraviolet absorbance of the column eluates and quantitation was achieved by measurement of the peak heights. The method showed linearity over a 100 fold range. The minimum detectable amounts ranged from 2 to 20 micrograms, depending on the compound. A test of these methods was performed by analyzing chicken plasma to which a mixture of chemicals was added. Recovery ranged from 30 to 70 percent. [Description provided by NIOSH]
• Subjects:
  Chromatography Energy Metabolism Hexanes Nervous System Occupational Health Polycyclic Aromatic Hydrocarbons Safety Solvents
• Keywords:
  Aromatic-hydrocarbons Chromatographic-analysis Metabolic-study Neurotoxic-effects NIOSH-Grant NIOSH-Publication
• ISSN:
  0003-2697
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  381-388
• Volume:
  151
• Issue:
  2
• NIOSHTIC Number:
  nn:00185848
• Citation:
  Anal Biochem 1985 Dec; 151(2):381-388
• Contact Point Address:
  Dr. M. B. Abou-Donia, Department of Pharmacology, Box 3813, Duke University Medical Center, Durham, N. C. 27710
• CAS Registry Number:
  2,5-Dimethylfuran (CAS RN 625-86-5) ; Cyclohexane (CAS RN 110-82-7) ; Hexane (CAS RN 110-54-3)
• Federal Fiscal Year:
  1986
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Analytical Biochemistry
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:54be6dedd48bd51c607e60aee9eb245aa9171f2331423ad8348cc18a600f4306b7a8d27484fba45e976ec1a709e3b76f8d1a734d1b7947049a7bb25891d4fcb9
• Download URL:
  https://stacks.cdc.gov/view/cdc/204161/cdc_204161_DS1.pdf
• File Type:
  [PDF - 632.37 KB ]