Inhalation of gas metal arc-stainless steel welding fume promotes lung tumorigenesis in A/J mice

Details

• Personal Author:
  Andrews R ; Antonini J ; Battelli L ; Cumpston A ; Cumpston J ; Erdely A ; Falcone L ; Kashon M ; McKinney W ; Meighan T ; Salmen R ; Stone S ; Zeidler-Erdely P
• Description:
  Epidemiologic studies suggest an increased risk of lung cancer with exposure to welding fumes, but controlled animal studies are needed to support this association. Oropharyngeal aspiration of gas metal arc-stainless steel (GMA-SS) welding fume has been shown by our laboratory to promote lung tumor formation in vivo using a two-stage initiation-promotion model. Our objective in this study was to determine if GMA-SS fume also acts as a lung tumor promoter when delivered via inhalation to lung tumor susceptible mice. Male A/J mice received intraperitoneal (IP) injections of corn oil or the chemical initiator 3-methylcholanthrene (MCA;10 microg/g) and one week later were exposed by whole body inhalation to air or GMA-SS welding aerosols for 4 h/d x 4 d/w x 9 w at a target concentration of 40 mg/m3. Lung nodules were enumerated at 30 weeks post-initiation. GMA-SS fume significantly promoted lung tumor multiplicity in A/J mice initiated with MCA (16.11 +/- 1.18) compared to MCA/air-exposed mice (7.93 +/- 0.82). Histopathological analysis found that the increased number of lung nodules in the MCA/ GMA-SS group were hyperplasias and adenomas, which was consistent with developing lung tumorigenesis. Lung metal deposition analysis revealed that a markedly lower deposited dose (approximately 5 fold) elicited a similar fold-change lung tumorigenic response when compared to our previous aspiration study. In conclusion, this study demonstrates that inhalation of GMA-SS welding fume promotes lung tumor formation in vivo and provides further support for the epidemiologic studies that show welders are at an increased risk for lung cancer. [Description provided by NIOSH]
• Subjects:
  Aerosols Carcinogens Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology Welding

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• Keywords:
  Inhalation Lung Cancer Stainless Steel Tumorigenesis Welding Fumes
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  156
• Issue:
  1
• NIOSHTIC Number:
  nn:20049460
• Citation:
  Toxicologist 2017 Mar; 156(1):327
• CAS Registry Number:
  Stainless Steel (CAS RN 12597-68-1)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 56th Annual Meeting and ToxExpo, March 12-16, 2017, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4b968e36bab086f189080d698c396a750f42cc7eed4222b876dc80f09f023776520a13559d0e5ef6ffe48e18905eedd77dbd0fb5606e9747efde1195a60dd024
• Download URL:
  https://stacks.cdc.gov/view/cdc/203904/cdc_203904_DS1.pdf
• File Type:
  [PDF - 951.12 KB ]