Detection of the antimicrobial triclosan in environmental samples by immunoassay

Details

• Personal Author:
  Ahn KC ; Bever CS ; Gee SJ ; Hammock BD ; Holland EB ; Hwang SH ; Morisseau K ; Pessah IN ; Ranganathan A
• Description:
  A sensitive, competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclosan (TCS; 2,4,4'-trichloro-2'-hydroxydiphenyl ether) was developed. Novel immunizing haptens were synthesized by derivatizing at the 4-Cl position of the TCS molecule. Compounds derived from substitutions at 4'-Cl and that replaced the 2'-OH with a Cl atom were designed as unique coating antigen haptens. Polyclonal rabbit antisera were screened against the coating antigen library to identify combinations of immunoreagents resulting in the most sensitive assays. The most sensitive assay identified was one utilizing antiserum no. 1155 and a heterologous competitive hapten, where the 2'-OH group was substituted with a Cl atom. An IC50 value and the detection range for TCS in assay buffer were 1.19 and 0.21-6.71 µg/L, respectively. The assay was selective for TCS, providing low cross-reactivity (<5%) to the major metabolites of TCS and to brominated diphenyl ether-47. A second assay utilizing a competitive hapten containing Br instead of Cl substitutions was broadly selective for both brominated and chlorinated diphenylethers. Using the most sensitive assay combination, we measured TCS concentrations in water samples following dilution. Biosolid samples were analyzed following the dilution of a simple solvent extract. The immunoassay results were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid and convenient tool to screen for human and environmental exposure. [Description provided by NIOSH]
• Subjects:
  Antibodies Energy Metabolism Environmental Monitoring Enzymes Occupational Health Safety
• Keywords:
  Antimicrobials ELISA Environmental Monitoring Enzyme-linked Immunosorbent Assay Exposure Assessment Immunoassays Metabolites Molecular Structure Screening Methods
• ISSN:
  0013-936X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  50
• Issue:
  7
• NIOSHTIC Number:
  nn:20049052
• Citation:
  Environ Sci Technol 2016 Apr; 50(7):3754-3761
• Contact Point Address:
  Bruce D. Hammock, Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States
• Email:
  bdhammock@ucdavis.edu
• CAS Registry Number:
  Triclosan (CAS RN 3380-34-5)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Environmental Science and Technology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:207c72bd9936b692e52c5158bf6ccca42f3335f7edfdf79df5e9c2eb7ca58452a88a25508d8ec32549993900e95b2a98b535155e76fece21f68f3698e820141b
• Download URL:
  https://stacks.cdc.gov/view/cdc/203633/cdc_203633_DS1.pdf
• File Type:
  [PDF - 699.63 KB ]