Pulmonary exposure to cellulose nanocrystals caused deleterious effects to reproductive system in male mice

Details

• Personal Author:
  Farcas MT ; Gutkin DW ; Kisin ER ; Menas AL ; Reiner RS ; Savolainen K ; Shvedova AA ; Star A ; Yanamala N
• Description:
  Over the past several years there has been an increased number of applications of cellulosic materials in many sectors, including the food industry, cosmetics, and pharmaceuticals. However, to date, there are few studies investigating the potential adverse effects of cellulose nanocrystals (CNC). The objective of this study was to determine long-term outcomes on the male reproductive system of mice upon repeated pharyngeal aspiration exposure to CNC. To achieve this, cauda epididymal sperm samples were analyzed for sperm concentration, motility, morphological abnormalities, and DNA damage. Testicular and epididymal oxidative damage was evaluated, as well as histopathology examination of testes. In addition, changes in levels of testosterone in testes and serum and of luteinizing hormone (LH) in serum were determined. Three months after the last administration, CNC exposure significantly altered sperm concentration, motility, cell morphology, and sperm DNA integrity. These parameters correlated with elevated proinflammatory cytokines levels and myeloperoxidase (MPO) activity in testes, as well as oxidative stress in both testes and epididymis. Exposure to CNC also produced damage to testicular structure, as evidenced by presence of interstitial edema, frequent dystrophic seminiferous tubules with arrested spermatogenesis and degenerating spermatocytes, and imbalance in levels of testosterone and LH. Taken together, these results demonstrate that pulmonary exposure to CNC induces sustained adverse effects in spermatocytes/spermatozoa, suggesting male reproductive toxicity. [Description provided by NIOSH]
• Subjects:
  Animals Cellulose Environmental Exposure Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Cellulose Fibers Cosmetics Industry Exposure Levels Histopathology Laboratory Animals Nanocrystals Pharmaceutical Industry Pulmonary Function Pulmonary System Pulmonary System Disorders Respiratory System Disorders Risk Factors Spermatozoa

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• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; OSHA Region 5 ; Pennsylvania ; West Virginia ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  79
• Issue:
  21
• NIOSHTIC Number:
  nn:20048547
• Citation:
  J Toxicol Environ Health A 2016 Aug; 79(21):984-997
• Contact Point Address:
  Anna A. Shvedova, Exposure Assessment Branch, NIOSH/CDC (MS-3030), 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  ats1@cdc.gov
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:18d91195c3065926efb82676652ab34beb43454d19f9da52bbf6a3464516751ed307d0f4bc9351b0eb9c5ace8430d2d3a253f596a92a702dc96cea9e579945b8
• Download URL:
  https://stacks.cdc.gov/view/cdc/203279/cdc_203279_DS1.pdf
• File Type:
  [PDF - 1.49 MB ]