Gender differences in murine pulmonary responses elicited by cellulose nanocrystals

Details

• Personal Author:
  Farcas MT ; Fournier PM ; Gutkin DW ; Halappanavar S ; Kagan VE ; Kisin ER ; Menas AL ; Reiner RS ; Reynolds JS ; Shvedova AA ; Star A ; Williams A ; Yanamala N
• Description:
  Background: Cellulose-based materials have been used for centuries to manufacture different goods derived from forestry and agricultural sources. In the growing field of nanocellulose applications, its uniquely engineered properties are instrumental for inventive products coming to competitive markets. Due to their high aspect ratio and stiffness, it is speculated that cellulose nanocrystals (CNC) may cause similar pulmonary toxicity as carbon nanotubes and asbestos, thus posing a potential negative impact on public health and the environment. Methods: The present study was undertaken to investigate the pulmonary outcomes induced by repeated exposure to respirable CNC. C57BL/6 female and male mice were exposed by pharyngeal aspiration to CNC (40 ug/mouse) 2 times a week for 3 weeks. Several biochemical endpoints and pathophysiological outcomes along with gene expression changes were evaluated and compared in the lungs of male and female mice. Results: Exposure to respirable CNC caused pulmonary inflammation and damage, induced oxidative stress, elevated TGF-â and collagen levels in lung, and impaired pulmonary functions. Notably, these effects were markedly more pronounced in females compared to male mice. Moreover, sex differences in responses to pulmonary exposure to CNC were also detected at the level of global mRNA expression as well as in inflammatory cytokine/chemokine activity. Conclusions: Overall, our results indicate that there are considerable differences in responses to respirable CNC based on gender with a higher pulmonary toxicity observed in female mice. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Cellulose Dust Environmental Exposure Environmental Monitoring Forestry Hazardous Substances Immune System Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Sex Characteristics Toxicology

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• Keywords:
  Agricultural Agricultural Industry Author Keywords: Cellulose Cell Alteration Cellulose-fibers Cytokines Dose Response Exposure-assessment Exposure Levels Gender Differences Gender Factors Genotoxic Effects Immune-reaction Inflammation Laboratory-animals Laboratory-testing Lung Function MRNA Expression Nanocellulose Nanocomposites Nanocrystals Nanotechnology Oxidative Stress Pulmonary-function Pulmonary-system-disorders Pulmonary Toxicity Respirable Dust Ribonucleic Acids Risk Factors RNA Toxic Effects Toxic Materials

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• ISSN:
  1743-8977
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; OSHA Region 5 ; Pennsylvania ; West Virginia ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  28
• Volume:
  13
• NIOSHTIC Number:
  nn:20048198
• Citation:
  Part Fibre Toxicol 2016 Jun; 13:28
• Contact Point Address:
  Anna A. Shvedova, Health Effects Laboratory Division, NIOSH, Exposure Assessment Branch, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  ats1@cdc.gov
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Particle and Fibre Toxicology
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:04e0c20f3d59028eb3a1607c46c7af5d6802a925f478987030d908232e666643142a33d415b0bcbe1cffeafa7fa4704305bd39340713a6bbc9e44ede1c68be66
• Download URL:
  https://stacks.cdc.gov/view/cdc/203014/cdc_203014_DS1.pdf
• File Type:
  [PDF - 2.19 MB ]