Single-cell mechanics provides an effective means to probe in vivo interactions between alveolar macrophages and silver nanoparticles

Details

• Personal Author:
  Anderson DS ; Karsai A ; Liu G-y ; Liu YX ; Pinderton KE ; Silva RM ; Uyeminami DL ; Van Winkle LS
• Description:
  Single-cell mechanics, derived from atomic force microscopy-based technology, provides a new and effective means to investigate nanomaterial-cell interactions upon in vivo exposure. Lung macrophages represent initial and important responses upon introducing nanoparticles into the respiratory tract, as well as particle clearance with time. Cellular mechanics has previously proven effective to probe in vitro nanomaterial-cell interactions. This study extends technology further to probe the interactions between primary alveolar macrophages (AM) and silver nanoparticles (AgNPs) upon in vivo exposure. Two types of AgNPs, 20 and 110 nm, were instilled to rat lung at 0.5 mg AgNPs/kg body weight, and allowed 24 h interaction. The consequences of these interactions were investigated by harvesting the primary AMs while maintaining their biological status. Cellular mechanics measurements revealed the diverse responses among AM cells, due to variations in AgNP uptake and oxidative dissolving into Ag+. Three major responses are evident: zero to low uptake that does not alter cellular mechanics, intracellular accumulation of AgNPs trigger cytoskeleton rearrangement resulting in the stiffening of mechanics, and damage of cytoskeleton that softens the mechanical profile. These effects were confirmed using confocal imaging of F-actin and measurements of reactive oxygen species production. More detailed intracellular interactions will also be discussed on the basis of this study in conjunction with prior knowledge of AgNP toxicity. [Description provided by NIOSH]
• Subjects:
  Animals Blood Cytotoxins Energy Metabolism Laboratories Lung Macrophages Metals Microbiology Occupational Health Oxidants Respiratory System Safety Silver

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• Keywords:
  Alveolar Cells Cell Function Cellular Reactions Cellular Structures Cellular Uptake Cytotoxicity Exposure Assessment In Vivo Study Laboratory Animals Lung Cells Metabolism Microscopic Analysis Microscopy Nanomaterials Nanoparticles Nanotechnology Oxidative Processes Reactive Oxygen Species Respiratory Function ROS Silver Compounds

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• ISSN:
  1089-5647
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  119
• Issue:
  49
• NIOSHTIC Number:
  nn:20048462
• Citation:
  J Phys Chem B 2015 Dec; 119(49):15118-15129
• Contact Point Address:
  G.-y. Liu, Department of Chemistry, School of Medicine, University of California, Davis, California 95616, United States
• Email:
  gyliu@ucdavis.edu
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Physical Chemistry B
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b8b08d096081aa2509e217546d076002bdc53851f13628deda3b28ef9e54c144f643302e00a8201eca499e6b050f72fd632e515b9f7eb972759c5665970b1f19
• Download URL:
  https://stacks.cdc.gov/view/cdc/203212/cdc_203212_DS1.pdf
• File Type:
  [PDF - 2.43 MB ]