Influence of particle size on persistence and clearance of aerosolized silver nanoparticles in the rat lung

Details

• Personal Author:
  Anderson DS ; Brown JM ; Chen LC ; Das GK ; Gordon T ; Guo T ; Patchin ES ; Pinkerton KE ; Shannahan J ; Sharmah A ; Silva RM ; Uyeminami DL ; Van Winkle LS
• Description:
  The growing use of silver nanoparticles (AgNPs) in consumer products raises concerns about potential health effects. This study investigated the persistence and clearance of 2 different size AgNPs (20 and 110 nm) delivered to rats by single nose-only aerosol exposures (6 h) of 7.2 and 5.4 mg/m(3), respectively. Rat lung tissue was assessed for silver accumulations using inductively-coupled plasma mass spectrometry (ICP-MS), autometallography, and enhanced dark field microscopy. Involvement of tissue macrophages was assessed by scoring of silver staining in bronchoalveolar lavage fluid (BALF). Silver was abundant in most macrophages at 1 day post-exposure. The group exposed to 20 nm AgNP had the greatest number of silver positive BALF macrophages at 56 days post-exposure. While there was a significant decrease in the amount of silver in lung tissue at 56 days post-exposure compared with 1 day following exposure, at least 33% of the initial delivered dose was still present for both AgNPs. Regardless of particle size, silver was predominantly localized within the terminal bronchial/alveolar duct junction region of the lung associated with extracellular matrix and within epithelial cells. Inhalation of both 20 and 110 nm AgNPs resulted in a persistence of silver in the lung at 56 days post-exposure and local deposition as well as accumulation of silver at the terminal bronchiole alveolar duct junction. Further the smaller particles, 20 nm AgNP, produced a greater silver burden in BALF macrophages as well as greater persistence of silver positive macrophages at later timepoints (21 and 56 days). [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Blood Cytotoxins Laboratories Lung Macrophages Metals Microbiology Occupational Health Particulate Matter Respiratory System Safety Silver

  More +
• Keywords:
  Aerosol Particles Alveolar Cells Author Keywords: Lung Bioaccumulation Cellular Reactions Cytotoxicity Dose Response Engineered Nanomaterials Exposure Assessment Inhalation Inhaled Laboratory Animals Lung Cells Lung Tissue Macrophage Mass Spectrometry Microscopic Analysis Microscopy Nanomaterials Nanoparticles Nanotechnology Particle Size Particle Size Analysis Pulmonary Clearance Silver Compounds

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; New York ; OSHA Region 2 ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  366-381
• Volume:
  144
• Issue:
  2
• NIOSHTIC Number:
  nn:20048463
• Citation:
  Toxicol Sci 2015 Apr; 144(2):366-381
• Contact Point Address:
  Laura S. Van Winkle, Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis, One Shields Avenue, Davis, California 95616
• Email:
  lsvanwinkle@ucdavis.edu
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Toxicological Sciences
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6e6bfde89c90eb9aa69f9b7d09198a821ad3df3123875a767dbd4475c6a46565e40836d5cec3a82d636cc699177dc44666c3bf4f21912b26234d6b622ea1aec4
• Download URL:
  https://stacks.cdc.gov/view/cdc/203213/cdc_203213_DS1.pdf
• File Type:
  [PDF - 1.07 MB ]