Gender differences in murine pulmonary responses elicited by nano-crystalline cellulose

Details

• Personal Author:
  Farcas MT ; Fournier PM ; Gutkin DW ; Halappanavar S ; Kagan VE ; Kisin ER ; Reiner R ; Sayre AL ; Shvedova AA ; Star A ; Yanamala N
• Description:
  The emerging field of nanotechnology attracts considerable attention to special properties of nanoparticles that are utilized in a number of novel products. Cellulose-based materials have been used for centuries to manufacture different goods derived from forestry and agricultural sources. In the growing field of nano-cellulose applications, its uniquely engineered properties (e.g. mechanical, thermal, rheological and optical) are instrumental for inventive products coming to competitive markets. Cellulose nanocrystals (CNC) is a crystalline form of cellulose obtained from different cellulose sources (bacterial, alga, wood pulp, cotton, etc.) by acid hydrolysis. CNCs are already produced on a large scale as nanofillers in polymer composites, building materials, cosmetics, food, and the drug industry. The high aspect ratio and stiffness of CNC may cause similar pulmonary toxicity as carbon nanotubes and asbestos thus posing a negative impact on public health and the environment. The present study was undertaken to investigate the pulmonary outcomes induced by repeated exposure to respirable CNC. C57BL6 female and male mice were treated by pharyngeal aspiration with CNC (40 microg/mouse) two times a week for three weeks. Exposure to respirable CNC caused pulmonary inflammation and damage, cytogenic alterations assessed by frequency of bi- and micro-nucleated cells in BAL, accelerated oxidative stress, elevated TGF-beta, collagen increase in lung and impaired pulmonary functions. Notably, these effects were markedly more pronounced in female compared to male mice. Moreover, gender differences in responses to pulmonary exposure to CNC were also detected at the level of global RNA expression as well as in cytokine/chemokine inflammatory reactions. Overall, our results indicate that there are considerable gender differences in responses to respirable CNC with a higher pulmonary toxicity observed in female mice. [Description provided by NIOSH]
• Subjects:
  Cellulose Dust Environmental Monitoring Hazardous Substances Immune System Laboratories Lung Occupational Health Polymers Respiratory System Respiratory Tract Diseases Safety Sex Characteristics Toxicology

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• Keywords:
  Cell Alteration Cellulose-fibers Cytokines Dose Response Exposure-assessment Gender Factors Gene Expression Genotoxic Effects Hydrolysis Immune-reaction Laboratory-animals Laboratory-testing Nanocellulose Nanocomposites Nanocrystals Nanotechnology Oxidative-processes Proteins Pulmonary-function Pulmonary-system-disorders Respirable Dust Ribonucleic Acids RNA Toxic Effects Toxic Materials

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 6 ; Pennsylvania ; West Virginia ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  68
• Volume:
  150
• Issue:
  1
• NIOSHTIC Number:
  nn:20047610
• Citation:
  Toxicologist 2016 Mar; 150(1):68
• CAS Registry Number:
  Cellulose (CAS RN 9004-34-6)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 55th Annual Meeting and ToxExpo, March 13-17, 2016, New Orleans, Louisiana
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c1321f9d7a36c7100cc6adb9b69ef809e3ab2f4fac104886f829cbdfb92ceeae3123bab85fdaa3aee1cf3b4d77a7568c51c6777d5e29cc008b9e06fbc7c410b1
• Download URL:
  https://stacks.cdc.gov/view/cdc/202625/cdc_202625_DS1.pdf
• File Type:
  [PDF - 442.29 KB ]