Computational dosimetry reveals the of role particles and ions in the toxicity of soluble silver nanoparticles

Details

• Personal Author:
  Jolley H ; Kodali VK ; Littke M ; Pounds JG ; Smith J ; Teeguarden JG ; Thomas DG ; Thrall BD
• Description:
  When suspended in cell culture media, nanomaterials composed of soluble metals such as silver can undergo dissolution resulting in ion formation and altered particle properties (e.g. mass, morphology, etc.) ultimately modulating cellular dose. Cultured cells are exposed not just to particles, but to a complex, dynamic mixture of particles, free ions, and ion-ligand complexes. Through a variety of nanoparticle dissolution, cellular uptake, and nanoparticle property experiments, a computational model (In Vitro Sedimentation, Diffusion, Dissolution, and Dosimetry Model (ISD3)) was developed to predict particle and ionic dosimetry to cells exposed to silver nanoparticles using a population balance approach. Using three different cell types (bone marrow derived macrophages from wild-type C57BL/6J mice and Scavenger Receptor A deficient mice and RAW 264.7 macrophages), ISD3 accurately predicts nanoparticle dissolution in cell culture media and silver dosimetry for two sizes of silver nanoparticles (20 and 110 nm) and silver ions. Toxicity to those three cell types were measured after exposure to different concentrations of two sizes of silver nanoparticles, and ISD3 was used to predict dosimetrics of nanoparticle exposure conditions. Multiple mixed linear regression model analyses suggest that intracellular nanoparticle surface area was the best predictor of cytotoxicity and viability of all experimental conditions (e.g. cell type, nanoparticle size, etc.). Further experimental results demonstrate that ions formed from nanoparticle dissolution may rapidly complex with ligands reducing toxicity. Overall these results suggest intracellular nanoparticle surface area is the most important determinate of silver nanoparticle toxicity, implicating intracellular dissolution as a potential mechanism. [Description provided by NIOSH]
• Subjects:
  Blood Cells, Cultured Chemistry Cytotoxins Laboratories Macrophages Metals Occupational Health Particulate Matter Radiation Dosimeters Safety Silver Toxicology

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• Keywords:
  Analytical Models Bone Marrow Cell Cultures Cell Culture Techniques Cell Type Cellular Uptake Chemical Properties Cytotoxicity Diffusion Analysis Dosimetry Laboratory Animals Laboratory Testing Mathematical Models Metal Compounds Models Morphology Nanomaterials Nanotechnology Particle Size Particulates Silver Compounds Soluble Surface Properties

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; OSHA Region 10 ; OSHA Region 3 ; OSHA Region 6 ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  122-123
• Volume:
  150
• Issue:
  1
• NIOSHTIC Number:
  nn:20047626
• Citation:
  Toxicologist 2016 Mar; 150(1):122-123
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2016
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 55th Annual Meeting and ToxExpo, March 13-17, 2016, New Orleans, Louisiana
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5ac84c76af38acef32103fe3e486ebe9c3cf7c53057783c073d5c5b296c272056a3c21275ddb5eeb00b75c6799553fb10137abe5e2ec6153f13b6afa7bf4cfd9
• Download URL:
  https://stacks.cdc.gov/view/cdc/202641/cdc_202641_DS1.pdf
• File Type:
  [PDF - 451.63 KB ]