Alteration of allergic response following an acute pulmonary exposure to nickel oxide nanoparticles in a murine OVA asthma model

Details

• Personal Author:
  Anderson, Stacey E. ; McLoughlin CE ; Roach KA ; Roberts, Jennifer R. ; Schwegler-Berry D ; Stefaniak, Aleksandr B.
• Description:
  Nickel is a causative agent of both dermal and respiratory allergy; however, despite the emerging nanotoxicological concept of size-dependent particle toxicity and increases in industrial use, nickel nanoparticles' effect on the immune system compared to larger nickel particles has not been explored. This study aimed to assess alterations in allergic disease following an acute exposure to NiO nanoparticles in an ovalbumin (OVA) allergy model, as compared to larger-sized NiO particles. Prior to in vivo exposures, two different forms of NiO particles were characterized in dispersion medium (DM: vehicle control). NiO particles had a primary size of < 5 microm diameter and served as a nickel control (NiO) and nano-sized NiO particles had a primary particle size approximately 50 nm diameter (NiON). On day 0, mice were oropharyngeally aspirated with 40 microg of NiO, NiON, or DM. On days 1 and 10, mice were sensitized to OVA via intraperitoneal injection and were challenged with OVA on days 19 and 28 via oropharyngeal aspiration. A separate group of particle-treated mice were not exposed to OVA and served as the non-allergic control groups. Following OVA-challenge on day 28, airway hyperreactivity (AHR) was assessed. On day 29, lungs were lavaged, IgE, cytokines, and lactate dehydrogenase activity were assessed in the fluid, and lavage cells were phenotyped. Serum and lung-associated lymph node cells were also collected for analysis. At day 29, there were no significant increases in lung injury or inflammation in mice exposed to NiO or NiON in the absence of OVA-induced allergy. OVA-sensitized animals treated with NiO did not exhibit any significant differences in allergic parameters when compared to OVA controls. However, AHR, lung-associated lymphocytes, and lung eosinophils were significantly increased in OVA-exposed animals treated with NiON when compared to the OVA control and the OVA-exposed NiO group. Overall, NiON, but not NiO, significantly altered the severity of allergic response in the OVA model following an acute exposure to particles prior to sensitization. These results suggest that exposure to a given concentration of nano-sized NiO may pose a greater health risk in populations susceptible to pulmonary allergic disease than that of its larger counterpart. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Hypersensitivity Immune System Laboratories Lung Lung Diseases Nickel Occupational Exposure Occupational Health Particulate Matter Respiratory System Safety Toxicology

  More +
• Keywords:
  Acute Exposure Airway-resistance Allergic-reactions Analytical-models Dispersion Dose-response Exposure-assessment Exposure-levels Immune-reaction Laboratory-animals Laboratory-testing Lung-cells Lung-disorders Lung-function Lymph-nodes Nanotechnology Nickel-compounds Particle Size Sensitization

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  150
• Issue:
  1
• NIOSHTIC Number:
  nn:20047625
• Citation:
  Toxicologist 2016 Mar; 150(1):120
• CAS Registry Number:
  Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 55th Annual Meeting and ToxExpo, March 13-17, 2016, New Orleans, Louisiana
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a69c990ec8ca9685f4f7398c97262d40b4ebcd939047315a0afaf78e948fd94138c8c8bc75ce26f431ce61661e71f5fe8866e4ca3fbc155b1b051d1731f71b00
• Download URL:
  https://stacks.cdc.gov/view/cdc/202640/cdc_202640_DS1.pdf
• File Type:
  [PDF - 441.95 KB ]