Lung toxicity and allergy responses in mice exposed to nanoparticle silver

Details

• Personal Author:
  Anderson KL ; Anderson S ; Chen BT ; McLoughlin CE ; Roach K ; Roberts, Jennifer R. ; Schwegler-Berry D
• Description:
  With expansive use of silver nanoparticles (AgNP) in medical applications and consumer products, potential for worker exposure during manufacturing has become a concern. The goal of the current study was to characterize the potential effects of AgNP in an ovalbumin (OVA)-induced allergy model in BALB/c mice. To characterize the effects of AgNP alone, mice were exposed via pharyngeal aspiration (PA) to physiological dispersion medium (DM), 6.1 microg, 18.2 microg, or 73 microg AgNP. Twenty nm diameter AgNP with 0.3% wt polyvinylpovidone coating (NanoAmor, Inc.) were suspended in DM and sonicated before exposures. For all studies lung function was assessed using enhanced pause (Penh); bronchoalveolar lavage (BAL) was performed on the whole lung; BAL cells and fluid were retained for analysis of lung-associated injury, inflammation, phenotyping; and lymph nodes (LN) were harvested for enumeration and immune cell phenotyping. AgNP alone did not result in changes in Penh, while cellular responses in the lung indicated a dose-dependent injury and inflammation by post-exposure day 10, which began to resolve by day 29. Our previous studies have shown that exposure to AgNP prior to OVA-sensitization results in a trend for the development of airway reactivity. In this study, effects of AgNP on the elicitation phase were examined. Animals were sensitized with i.p. injections of OVA (dose) + aluminum hydroxide gel on days 1 and 10. To elicit an OVA-specific response, two PA challenges with OVA were given on days 19 and 28. AgNP were administered by PA on day 27. AgNP did not appear to significantly enhance Penh, and lung-associated LN total cell numbers, BAL cell numbers and IgE levels in serum were not increased above those of the allergy model control (OVA). The results indicate that although AgNP may have a moderate effect on airway resistance in the lung when administered before sensitization, they do not significantly alter the course of allergy development when given either prior to sensitization or during the elicitation phase. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Hypersensitivity Immune System Laboratories Lung Lung Diseases Occupational Health Respiratory System Safety Silver Toxicology

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• Keywords:
  Airway-resistance Allergic-reactions Analytical-models Dispersion Dose-response Exposure-assessment Exposure-levels Immune-reaction Laboratory-animals Laboratory-testing Lung-cells Lung-disorders Lung-function Lymph-nodes Nanotechnology Sensitization Silver-compounds

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  144
• Issue:
  1
• NIOSHTIC Number:
  nn:20045898
• Citation:
  Toxicologist 2015 Mar; 144(1):144
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:36c0efecde16894a4c7da749c9744fa5b0d413dfec4d196e4691322ef448973a51bb921bb2418c6db42771d30d7d3b6601170fdc782c96eacfe616d4781fff2d
• Download URL:
  https://stacks.cdc.gov/view/cdc/201143/cdc_201143_DS1.pdf
• File Type:
  [PDF - 218.87 KB ]