Differences in nickel oxide nanoparticle-induced pulmonary toxicity and exacerbated allergic response following acute respiratory exposure

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• Personal Author:
  Anderson, Stacey E. ; McLoughlin CE ; Roach KA ; Roberts, Jennifer R. ; Schwegler-Berry D ; Stefaniak, Aleksandr B.
• Description:
  Particle size and morphology play critical roles in nanomaterial-induced lung inflammation, but the relationship of these factors to augmentation of allergic response in the respiratory tract is largely unknown. To address this concept, two different sizes of nickel oxide (NiO) particles were characterized and investigated in vivo. Dynamic light scattering showed the average particle sizes (APS) were 27 nm for NiO-1 and 190 nm for NiO-2. NiO-1 particles were spherical while NiO-2 particles were more irregular and plate-like. The goal of the study was to assess effects of the different NiO particles on augmentation of allergic response using an ovalbumin (OVA) model. Effects of NiO on the lung were also assessed at critical time points correlating to the OVA model in the absence of OVA. Female BALB/c mice were given a single dose of 40 micrograms of NiO-1, NiO-2, or dispersion medium (DM; vehicle control) by oropharyngeal aspiration (OPA) and euthanized at 1, 10, 19, and 29 d post-exposure in the absence of OVA. In the OVA allergy model, mice were similarly exposed to particles or DM on day 0, sensitized to OVA via i.p. injection at 1 and 10 d, challenged with OVA at 19 and 28 d via OPA, and euthanized at 29 d. In the absence of OVA, only NiO-2 induced significant and persistent increases in lung injury and inflammation in the lung, but both NiO-1 and NiO-2 increased mediastinal lymph node (LN) size as compared to controls. In the OVA allergy model, the smaller nanoparticles (NiO-1), resulted in an exacerbated airway response following OVA challenge, and increased serum OVA-specific IgE levels as compared to vehicle and allergy controls. Differentially, exposure to NiO-2 significantly increased LN size, yet reduced OVA-specific IgE levels. Overall, results demonstrate that size, and possibly particle morphology, contributed to nickel-based particle-induced pulmonary inflammation and modulation of immune responses in the lung. The larger plate-like particle was capable of inducing a greater degree of pulmonary inflammation; however, the smaller NiO particle exacerbated the allergic response to OVA to a greater degree. Further studies are needed to elucidate the mechanisms related to these findings. [Description provided by NIOSH]
• Subjects:
  Albumins Antibodies Environmental Exposure Hypersensitivity Immune System Laboratories Lung Lung Diseases Lymphatic System Occupational Exposure Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Acute Exposure Albumin Allergic Reactions Analytical Models Exposure Assessment Exposure Levels Immune Reaction Immune System Immunoglobulins In Vivo Study Laboratory Animals Laboratory Testing Lung Disorders Lung Irritants Lymph Nodes Morphology Nanomaterials Nanoparticles Nanotechnology Nanotoxicology Nickel Oxide Particle Size Pulmonary Effects Pulmonary System Disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  78-79
• Volume:
  156
• Issue:
  1
• NIOSHTIC Number:
  nn:20049387
• Citation:
  Toxicologist 2017 Mar; 156(1):78-79
• CAS Registry Number:
  Nickel monoxide (CAS RN 1313-99-1)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 56th Annual Meeting and ToxExpo, March 12-16, 2017, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9e225c50487a2bf7a6d87413f41fcdfa12df43ea77199b141be0018fcde95154cf285b8cdf0585b4e20ce19d25e396bb95a827142a0224d231549ae8d767eaac
• Download URL:
  https://stacks.cdc.gov/view/cdc/203847/cdc_203847_DS1.pdf
• File Type:
  [PDF - 964.97 KB ]