Toxicological assessment of CoO and La2O3 metal oxide nanoparticles in human small airway epithelial cells

Details

• Personal Author:
  Andrew, Michael E. ; Castranova, Vincent ; Chisholm WP ; Demokritou P ; Mihalchik AL ; Pirela SV ; Qian Y ; Schwegler-Berry D ; Shaffer J ; Sisler JD
• Description:
  Cobalt monoxide (CoO) and lanthanum oxide (La2O3) nanoparticles are two metal oxide nanoparticles with different redox potentials according to their semiconductor properties. By utilizing these two nanoparticles, this study sought to determine how metal oxide nanoparticle's mode of toxicological action is related to their physio-chemical properties in human small airway epithelial cells (SAEC). We investigated cellular toxicity, production of superoxide radicals and alterations in gene expression related to oxidative stress and cellular death at 6 and 24 h following exposure to CoO and La2O3 (administered doses: 0, 5, 25, and 50 ug/ml) nanoparticles. CoO nanoparticles induced gene expression related to oxidative stress at 6 h. After characterizing the nanoparticles, transmission electron microscope (TEM) analysis showed SAEC engulfed CoO and La2O3 nanoparticles. CoO nanoparticles were toxic after 6 h and 24 h of exposure to 25.0 and 50.0 ug/ml administered doses, whereas, La2O3 nanoparticles were toxic only after 24 h using the same administered doses. Based upon the Volumetric Centrifugation Method in vivo Sedimentation, Diffusion and Dosimetry (VCM-ISDD), the dose of CoO and La2O3 nanoparticles delivered at 6 and 24 h were determined to be: CoO: 1.25, 6.25, and 12.5 ug/ml; La2O3: 5, 25, and 50 ug/mland CoO: 4, 20, and 40 ug/ml; and La2O3: 5, 25, 50 ug/ml respectively. CoO nanoparticles produced more superoxide radicals and caused greater stimulation of total tyrosine and threonine phosphorylation at both 6 h and 24 h when compared to La2O3 nanoparticles. Taken together, these data provide evidence that different toxicological modes of action were involved in CoO and La2O3 metal oxide nanoparticle-induced cellular toxicity. [Description provided by NIOSH]
• Subjects:
  Chemistry Cytotoxins Genetics Hazardous Substances Metals Occupational Health Safety Toxicology
• Keywords:
  Author Keywords: Cell Culture Cell-function Cellular-function Chemical-properties Cytotoxicity Genes Metal-oxides Metal Oxides Nanoparticles Nanotechnology Oxidative-processes Oxidative Stress Physical-properties Stress Toxic-effects Toxins

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  418-428
• Volume:
  150
• Issue:
  2
• NIOSHTIC Number:
  nn:20047404
• Citation:
  Toxicol Sci 2016 Apr; 150(2):418-428
• Contact Point Address:
  Dr. Yong Qian, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  yaq2@cdc.gov
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fcab4607bac51bb2cdd86943dd057a3c324d76dc26644beaedc86bcc646fedda121c566203f9b0741e989694ebb6aadad2dd027275ae741c0eb28b64309565f1
• Download URL:
  https://stacks.cdc.gov/view/cdc/202479/cdc_202479_DS1.pdf
• File Type:
  [PDF - 667.64 KB ]