Dermal Toxicity of Nickel- and Cobalt-Based Nanocatalysts

Details

• Personal Author:
  Guppi S ; Khaliullin T ; Kisin ER ; Shvedova AA ; Yanamala N
• Description:
  Nanocatalysis is a fast-growing field involving the use of nanomaterials as catalysts for a variety of applications, specifically, metal nanoparticles (NP) and their compounds, which have a large surface-to-volume ratio compared to bulk materials. Metal/metal oxide (Me/MeO) NP possess unique properties which can be useful in different applications including catalytic processes such as decomposition, reactions of dehydrogenation, oxidation, alkylation, C-C coupling, among others. Nonetheless, the same properties that make these metal nanocatalysts (NCT) very attractive can pose potential health risks. In addition to inhalation exposure route, workers may also be exposed through skin contact. In this study, we evaluated the ability of four different NCT (NiFe2O4, CoFe2O4, Ni and Co3O4) to initiate oxidative stress, induce redox-sensitive transcription factors and to trigger inflammation in primary human epidermal keratinocytes (HEK). Besides, due to the skin's vulnerability to UV radiation, it is important to assess whether metal NCT augment the adverse effects of UVB. HEKs exposure to the studied Me/MeO NCT (0-20 microg/cm2) resulted in a dose- and time-dependent reduction in cell viability, cell damage, activation of NF-kappaB, elevated ROS generation, release of inflammatory mediators, and increase in oxidative stress markers. Co-exposure of HEK to UVB (4KJ/m2) and Me/MeO caused marked amplification of the observed responses. UVB exposure alone induced significant cytotoxicity and secretion of cytokines/chemokines. Based on the hierarchical clustering analysis of the cytokine/chemokine responses, Co3O4 and Ni were segregated from the control and both ferrites-exposed samples. Pre-treatment with UVB resulted in separation of Ni, Co3O4 and NiFe2O4 responses from control and CoFe2O4 exposure groups. Overall the inflammatory responses in HEK cells induced by exposure to different Me/MeO NPs investigated, with or without UVB pre-treatment, were in order: Ni>Co3O4>NiFe2O4>CoFe2O4. Altogether, these data indicated that co-exposure of dermal cells in vitro to Me/MeO NP and UVB was associated with potentiation of the adverse effects as compared to the cells treated with NCT alone. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Chemistry Cytotoxins Hazardous Substances Immune System Metals Occupational Health Safety Skin Toxicology
• Keywords:
  Catalysts Cell Cultures Cell Culture Techniques Cell Damage Cellular Reactions Chemical Processing Chemical Properties Chemical Reactions Crystal Structure Cytokines Cytotoxicity Dermal Exposures Exposure Assessment Gene Expression Immune Reaction In Vitro Study Metal Compounds Metal Oxides Nanomaterials Nanotoxicology Oxidation Reduction Reactions Oxidative Stress Skin Exposure Toxic Effects Toxic Materials Ultraviolet Radiation

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058939
• Citation:
  Toxicologist 2020 Mar; 174(1):267
• CAS Registry Number:
  Cobalt (CAS RN 7440-48-4) ; Cobalt oxide (Co3O4) (CAS RN 1308-06-1) ; Iron (CAS RN 7439-89-6) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:400356075e4bd07da8cb83bad32313ff8fa178e4d7f3d3100002838defec76780acf47569419a97dc2db66d50be3501a4a72606579e7fddecbfd994aa1db955f
• Download URL:
  https://stacks.cdc.gov/view/cdc/223294/cdc_223294_DS1.pdf
• File Type:
  [PDF - 522.74 KB ]