Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns

Details

• Personal Author:
  Bailey KL ; Cloonan D ; DeVasure JM ; Romberger DJ ; Schneberger D ; Wyatt TA
• Description:
  Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9% CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Carbon Dioxide Dust Genetics Immune System Lung Occupational Health Pathology Respiratory System Safety

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• Keywords:
  Agricultural Workers Alveolar Cells Animal Husbandry Workers Author Keywords: Hypercapnia Barn Dust Bronchial Epithelium Carbon Dioxide Dust Exposure Genes Immune Reaction LPS Lung Tissue Organic Dusts TLR

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• ISSN:
  1567-5769
• Document Type:
  Text
• Funding:
  Grant ; Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  76-84
• Volume:
  27
• Issue:
  1
• NIOSHTIC Number:
  nn:20047341
• Citation:
  Int Immunopharmacol 2015 Jul; 27(1):76-84
• Contact Point Address:
  Todd A. Wyatt, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, 985910 The Nebraska Medical Center, Omaha, NE 68198-5910, United States
• Email:
  twyatt@unmc.edu
• CAS Registry Number:
  Carbon dioxide (CAS RN 124-38-9)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  International Immunopharmacology
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1ee5b0d585d1aa5f64c86f52a34359cd63563da825faa6fcbf99587a2626231a7f28c72299ebf703165f77a304b8b1d95872345774feb7eba8bd2d7edb3a57e5
• Download URL:
  https://stacks.cdc.gov/view/cdc/202446/cdc_202446_DS1.pdf
• File Type:
  [PDF - 736.24 KB ]