Novel antigens of Mycobacterium immunogenum relevant in serodiagnosis of occupational hypersensitivity pneumonitis in machinists

Details

• Personal Author:
  Chandra H ; Lockey J ; Yadav JS
• Description:
  Hypersensitivity pneumonitis (HP) in machinists is a chronic interstitial lung disease associated with exposures to in-use metal working fluid, particularly fluid contaminated with nontuberculous mycobacteria species Mycobacterium immunogenum (MI). In this context, MI also has been shown to induce HP-like pathology in mice. The general assumption has been that preformed antigens (and not a live infection) are responsible for inducing HP. However, a recent clinical study reported culturing of MI from the sputum of a metalworker. Our recent study using a murine alveolar macrophage cell line demonstrated that MI can multiply intracellularly and induce inflammatory mediators implicated in HP.6 Hence, machinists repeatedly exposed to the inciting mycobacterial antigens, preformed or generated de novo during infection, are at risk for developing HP. Despite recognition of the mycobacterial etiology, there are no reliable serodiagnostic methods currently available for HP. Detection of serum immunoglobulins (eg, IgG) to the etiologic agent can be useful if their critical antigens are identified. In this direction, our first immuno-proteomic study on MI led to the identification of 33 immunoreactive proteins, including 4 secretory, 6 associated with the cell wall, 11 membranous, and 12 cytosolic. In the present study, we generated soluble recombinant forms of 3 cell wallassociated proteins (arbitrarily designated antigens A through C [Ag A through Ag C]) and 2 secretory proteins (Ag D and Ag E) in Escherichia coli Rosetta Blue (DE3) cells. All 5 recombinant proteins were purified to homogeneity by the nickel and nitrilotriacetic acid method and made free from endotoxin. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Fungi Laboratories Lubricants Manufacturing And Industrial Facilities Metals Metal Workers Microbiology Occupational Health Risk Assessment Risk Factors Safety Workplace

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• Keywords:
  Bacteria Cell-biology Cell-function Cellular-function Cellular-reactions Diseases Exposure-levels Laboratory-animals Machinists Metal-compounds Metal-workers Metallic-compounds Metallurgical-processes Metallurgy Metalworking-fluids Pathogenicity Risk-factors Work-environment Workers

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• ISSN:
  1081-1206
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  114
• Issue:
  6
• NIOSHTIC Number:
  nn:20046680
• Citation:
  Ann Allergy Asthma Immunol 2015 Jun; 114(6):525-526.e4
• Contact Point Address:
  Jagjit S. Yadav, PhD, Division of Environmental Genetics and Molecular Toxicology, Division of Occupational and Environmental Medicine, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267
• Email:
  jagjit.yadav@uc.edu
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  Annals of Allergy, Asthma and Immunology
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0c5ef680fe8f5d24f4aba2c955e8c31eb8668b93fbfee606e6ba0d85c9da5fa1ec1ca79676cfd879f5ce912aa15a9090b15a4152d1ef06547f44b33b21b35847
• Download URL:
  https://stacks.cdc.gov/view/cdc/202032/cdc_202032_DS1.pdf
• File Type:
  [PDF - 885.31 KB ]