Extended tracking of the microbial community structure and dynamics in an industrial synthetic metalworking fluid system

Details

• Personal Author:
  Kapoor R ; Selvaraju SB ; Yadav JS
• Description:
  Understanding of the occupational exposure risk scenario and disease etiology associated with industrial metalworking fluids (MWFs) requires knowledge of the development and composition of their microbial diversity in relation to the underlying fluid management factors. In this study, a managed synthetic MWF operation freshly recharged following the dumping, cleaning, and recharge (DCR) process was tracked in real time for microbial community changes over a period of 1.25 years (65 weeks). The recharged fluid developed very high bacterial counts (viable and nonviable) fairly quickly after the DCR process, indicating its inadequacy. Genus-/group-specific real-time qPCR confirmed the prevalence of six potentially pathogenic/immunogenic microbial genera/groups, viz. pseudomonads, enterics, mycobacteria, legionellae, actinomycetes, and fungi. Selective culturing revealed Acinetobacter and Bacillus as the most frequently isolated Gram-negative and Gram-positive genera, respectively, in addition to the presence of fungi and actinomycetes. Endotoxin perturbations (< 1000 to > 100000 EU mL?¹) coincided with temporal increases in Gram-negative bacteria and/or periodic biocide additions. PCR-DGGE-sequencing revealed an expanded estimated bacterial richness (up to 23 bands per sample). Of the 16 dominant bacterial phylotypes identified, the majority were detected for the first time in MWF. Interestingly, the study revealed a crucial role for MWF brand, among other fluid factors, in modulating the community structure and dynamics. [Description provided by NIOSH]
• Subjects:
  Endotoxins Fungi Lubricants Metals Microbiology Occupational Health Risk Assessment Risk Factors Safety
• Keywords:
  Author Keywords: Metalworking Fluid Bacteria DGGE Diseases Endotoxin Etiology Exposure-levels Metal-compounds Metallic-compounds Metallurgical-processes Metallurgy Metalworking-fluids Pathogenicity Real-time QPCR Risk-factors

  More +
• ISSN:
  0168-6496
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  87
• Issue:
  3
• NIOSHTIC Number:
  nn:20044569
• Citation:
  FEMS Microbiol Ecol 2014 Mar; 87(3):664-677
• Contact Point Address:
  Jagjit S. Yadav, Department of Environmental Health, University of Cincinnati, College of Medicine, Cincinnati, OH 45267-0056
• Email:
  jagjit.yadav@uc.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  FEMS Microbiology Ecology
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6a8b935ba029341a11f65933dc233b493b7bcd091a0dbcf58f4f288602bf75601959265a62a951592083d87d938ac2796f7ecb6485fbcfe181dcb3edc0bb2dad
• Download URL:
  https://stacks.cdc.gov/view/cdc/200243/cdc_200243_DS1.pdf
• File Type:
  [PDF - 568.61 KB ]